WormBase Tree Display for Variation: WBVar00000484
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| WBVar00000484 | Evidence | Paper_evidence | WBPaper00002282 | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Name | Public_name | bn74 | |||||||
| Sequence_details | Mapping_target | F54F7 | |||||||
| Type_of_mutation | Deletion | ||||||||
| SeqStatus | Pending_curation | ||||||||
| Variation_type | Allele | ||||||||
| Origin | Species | Caenorhabditis elegans | |||||||
| Laboratory | SS | ||||||||
| Positive_clone | C38D5 | ||||||||
| Status | Live | ||||||||
| Affects | Gene | WBGene00001002 | |||||||
| WBGene00003219 | |||||||||
| Interactor (25) | |||||||||
| Genetics | Mapping_data | In_multi_point | 2169 | ||||||
| 2172 | |||||||||
| Description | Phenotype | WBPhenotype:0000436 | Paper_evidence | WBPaper00031440 | |||||
| WBPaper00005375 | |||||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| WBPerson2987 | |||||||||
| Remark | MEG-1 and PGL-1 fail to tightly colocalize in mes-1 embryos | Paper_evidence | WBPaper00031440 | ||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| "Consistent with the idea that MES-1 is required in both cells, the junctional MES-1 staining was not restored if MES-1 was absent in either P2 or EMS (Figures 4I and 4J)." | Paper_evidence | WBPaper00005375 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Variation_effect | Null | Paper_evidence | WBPaper00031440 | ||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| EQ_annotations | Anatomy_term | WBbt:0006876 | PATO:0000460 | Paper_evidence | WBPaper00005375 | ||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBbt:0006873 | PATO:0000460 | Paper_evidence | WBPaper00005375 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Life_stage | WBls:0000003 | PATO:0000460 | Paper_evidence | WBPaper00031440 | |||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Temperature_sensitive | Heat_sensitive | Paper_evidence | WBPaper00031440 | ||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Maternal | Strictly_maternal | Paper_evidence | WBPaper00031440 | ||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Phenotype_assay | Treatment | MEG-1 antibody staining | Paper_evidence | WBPaper00031440 | |||||
| Curator_confirmed | WBPerson2021 | ||||||||
| Temperature | 25C | Paper_evidence | WBPaper00031440 | ||||||
| Curator_confirmed | WBPerson2021 | ||||||||
| WBPhenotype:0001133 | Paper_evidence | WBPaper00005375 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "Interestingly, we found that mes-1(+) activity was required in both EMS and P2 to orient the mitotic spindle of EMS (Figures 4E and 4F)." | Paper_evidence | WBPaper00005375 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| EQ_annotations | Anatomy_term | WBbt:0006876 | PATO:0000460 | Paper_evidence | WBPaper00005375 | ||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0001351 | Paper_evidence | WBPaper00005375 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "We found that throughout the four-cell stage mes-1 mutants exhibited normal levels of pTyr staining at other cell junctions but exhibited a reduced level of staining at the P2/EMS junction (compare Figures 3E and 3F to Figures 3G and 3H). Likewise, pTyr staining was reduced at the P3/E and P4/Ep contact sites (data not shown)." | Paper_evidence | WBPaper00005375 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0001504 | Person_evidence | WBPerson261 | |||||||
| Curator_confirmed | WBPerson712 | ||||||||
| Remark | ts impenetrant Mes, resembling bn7 | Person_evidence | WBPerson261 | ||||||
| Curator_confirmed | WBPerson712 | ||||||||
| Temperature_sensitive | Heat_sensitive | 25 | Person_evidence | WBPerson261 | |||||
| Curator_confirmed | WBPerson712 | ||||||||
| WBPhenotype:0001681 | Paper_evidence | WBPaper00005375 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "Also as observed in src-1 mutants, we found that 63% (n = 27) of mes-1 mutant embryos exhibited an initially skewed alignment of the EMS spindle." | Paper_evidence | WBPaper00005375 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| EQ_annotations | Anatomy_term | WBbt:0006876 | PATO:0000460 | Paper_evidence | WBPaper00005375 | ||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Phenotype_not_observed | WBPhenotype:0001133 | Paper_evidence | WBPaper00005375 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | "we did not observe L/R EMS divisions in the mes-1 mutant embryos examined (Table 1)." | Paper_evidence | WBPaper00005375 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| EQ_annotations | Anatomy_term | WBbt:0006876 | PATO:0000460 | Paper_evidence | WBPaper00005375 | ||||
| Curator_confirmed | WBPerson2987 | ||||||||
| WBPhenotype:0001637 | Paper_evidence | WBPaper00005375 | |||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Remark | Table 1 | Paper_evidence | WBPaper00005375 | ||||||
| Curator_confirmed | WBPerson2987 | ||||||||
| EQ_annotations | Anatomy_term | WBbt:0005772 | PATO:0000460 | Paper_evidence | WBPaper00005375 | ||||
| Curator_confirmed | WBPerson2987 | ||||||||
| Reference | WBPaper00005375 | ||||||||
| WBPaper00031440 | |||||||||
| Remark | bn74 comprises a 25 kb deletion that removes the entire coding region mes-1. Exact breakpoints are not detailed in the paper. | Paper_evidence | WBPaper00002282 | ||||||
| Method | Deletion_allele |
