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WormBase Tree Display for Variation: WBVar00088539

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Name Class

WBVar00088539EvidencePaper_evidenceWBPaper00002935
NamePublic_namem27
Other_name (22)
HGVSgCHROMOSOME_I:g.10773623G>A
Sequence_detailsSMapS_parentSequenceR13H8
Flanking_sequencesgatatctatgatgatctagaattcccatcatggttggcgaatcggttccagcaattccaagt
Mapping_targetR13H8
Type_of_mutationSubstitutiongaPaper_evidenceWBPaper00002935
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00006157
WBStrain00006233
LaboratoryDR
StatusLive
AffectsGeneWBGene00000912
Transcript (11)
Interactor (11)
GeneticsInterpolated_map_positionI5.111
Mapping_dataIn_2_point442
In_multi_point336
3631
DescriptionPhenotypeWBPhenotype:0000013Paper_evidenceWBPaper00000316
WBPaper00000504
Curator_confirmedWBPerson712
WBPhenotype:0000304Paper_evidenceWBPaper00004310
Curator_confirmedWBPerson2021
RemarkAnimals exhibit severely reduced responses to isoamyl alcohol (data not shown)Paper_evidenceWBPaper00004310
Curator_confirmedWBPerson2021
Affected_byMoleculeWBMol:00001063Paper_evidenceWBPaper00004310
Curator_confirmedWBPerson2021
WBPhenotype:0000480Paper_evidenceWBPaper00004310
Curator_confirmedWBPerson2021
RemarkAnimals exhibit significantly reduced responses to pyrazine (data not shown)Paper_evidenceWBPaper00004310
Curator_confirmedWBPerson2021
Affected_byMoleculeWBMol:00005360Paper_evidenceWBPaper00004310
Curator_confirmedWBPerson2021
WBPhenotype:0000731Paper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
RemarkWeak increase in levels of apoptosis in daf-16 loss-of-function mutants treated with IRPaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Variation_effectProbable_null_via_phenotypePaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
EQ_annotationsAnatomy_termWBbt:0006796PATO:0000460Paper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Life_stageWBls:0000041PATO:0000460Paper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentTreated with 60 Gy IRPaper_evidenceWBPaper00029085
Curator_confirmedWBPerson2021
WBPhenotype:0001085Paper_evidenceWBPaper00004310
Curator_confirmedWBPerson2021
RemarkAnimals exhibit severely reduced responses to butanone (data not shown)Paper_evidenceWBPaper00004310
Curator_confirmedWBPerson2021
Affected_byMoleculeWBMol:00005086Paper_evidenceWBPaper00004310
Curator_confirmedWBPerson2021
WBPhenotype:0001086Paper_evidenceWBPaper00004310
Curator_confirmedWBPerson2021
RemarkAnimals exhibit significantly reduced responses to trimethylthiazole (data not shown)Paper_evidenceWBPaper00004310
Curator_confirmedWBPerson2021
Affected_byMoleculeWBMol:00004538Paper_evidenceWBPaper00004310
Curator_confirmedWBPerson2021
WBPhenotype:0001171Paper_evidenceWBPaper00031942
Curator_confirmedWBPerson712
RemarkAnimals exhibited a slightly shorter life span compared to wild-type animals.Paper_evidenceWBPaper00031942
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were grown in microfuge tubes in 100l S medium (maintained) and incubated in thermocyclers. Worms were transferred into autoclaved, sterile microfuge tubes either every other day or once every 3 days, dependent upon the strain and stage of life of the worms used. Worms were monitored, counted and fed on a daily basis. Animals were shifted every 10 minutes between 12C and 25C.Paper_evidenceWBPaper00031942
Curator_confirmedWBPerson712
Temperature12, 12.5, 18.5, 25Paper_evidenceWBPaper00031942
Curator_confirmedWBPerson712
WBPhenotype:0001775Paper_evidenceWBPaper00031942
Curator_confirmedWBPerson712
RemarkUnlike wild-type, growth under thermocycling conditions of 10 minute shifts between 12C and 25C, resulted in a shift in life span similar to the life span of animals reared at 18.5C continuously, not towards animals reared at 12C, as is the response of wild-type animals.Paper_evidenceWBPaper00031942
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were grown in microfuge tubes in 100l S medium (maintained) and incubated in thermocyclers. Worms were transferred into autoclaved, sterile microfuge tubes either every other day or once every 3 days, dependent upon the strain and stage of life of the worms used. Worms were monitored, counted and fed on a daily basis. Animals were shifted every 10 minutes between 12C and 25C.Paper_evidenceWBPaper00031942
Curator_confirmedWBPerson712
Temperature12, 12.5, 18.5, 25Paper_evidenceWBPaper00031942
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0000147Paper_evidenceWBPaper00040181
Curator_confirmedWBPerson712
Remarkdaf-16(m27) mutant worms responded to starvation as did wild-type N2 worms in both lpd-7 expression and rRNA synthesis.Paper_evidenceWBPaper00040181
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000056PATO:0000460Paper_evidenceWBPaper00040181
Curator_confirmedWBPerson712
WBPhenotype:0000481Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
RemarkMutant animals did not display an abnormal aversion response to copper, compared to wild type animals (Figure 2B)Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00002862Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
WBPhenotype:0001470Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
RemarkMutants exhibited no change in chemotaxis towards diacetyl, compared to wild type controls (Figure 2A)Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00002819Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
WBPhenotype:0001789Paper_evidenceWBPaper00031942
Curator_confirmedWBPerson712
RemarkAnimals displayed basically the same overall pattern as observed with wild-type animals in that animals were short lived at 25C, intermediate at 18.5C and long lived at 12.5C.Paper_evidenceWBPaper00031942
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were grown in microfuge tubes in 100l S medium (maintained) and incubated in thermocyclers. Worms were transferred into autoclaved, sterile microfuge tubes either every other day or once every 3 days, dependent upon the strain and stage of life of the worms used. Worms were monitored, counted and fed on a daily basis. Animals were shifted every 10 minutes between 12C and 25C.Paper_evidenceWBPaper00031942
Curator_confirmedWBPerson712
Temperature12, 12.5, 18.5, 25Paper_evidenceWBPaper00031942
Curator_confirmedWBPerson712
WBPhenotype:0001999Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
RemarkThe integration of signals for attraction to diacetyl (100x dilute) and avoidance from copper (100 millimolar) was normal (like wild type) in daf-16(m27) insulin-like signaling pathway mutants, resulting in the same number of animals crossing the copper barrier to get to the diacetyl spot compared to wild type controls (Figure 1C)Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00002862Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
WBMol:00002819Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
WBPhenotype:0002535Paper_evidenceWBPaper00000932
Curator_confirmedWBPerson712
RemarkFITC uptake is normal.Paper_evidenceWBPaper00000932
Curator_confirmedWBPerson712
WBPhenotype:0004023Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
RemarkMutant animals exhibit a wild type frequency of body bends (Figure 3A)Paper_evidenceWBPaper00037970
Curator_confirmedWBPerson2987
Reference (13)
MethodSubstitution_allele