Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Variation: WBVar00088567

expand all nodes | collapse all nodes | view schema

Name Class

WBVar00088567EvidencePaper_evidenceWBPaper00003883
NamePublic_namem77
Other_nameF01G10.8a.1:c.574C>T
F01G10.8b.1:c.706C>T
CE29090:p.Gln192Ter
CE45907:p.Gln236Ter
HGVSgCHROMOSOME_IV:g.10254765C>T
Sequence_detailsSMapS_parentSequenceF01G10
Flanking_sequencesaataagccactgaaatatgtgtttcggttgaaaacaaaggagatcaaaaaagaatgaaaa
Mapping_targetF01G10
Type_of_mutationSubstitutionctPaper_evidenceWBPaper00003883
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
Strain (12)
LaboratoryDR
GOU
StatusLive
AffectsGeneWBGene00000910
TranscriptF01G10.8b.1VEP_consequencestop_gained
VEP_impactHIGH
HGVScF01G10.8b.1:c.706C>T
HGVSpCE45907:p.Gln236Ter
cDNA_position725
CDS_position706
Protein_position236
Exon_number6/8
Codon_changeCaa/Taa
Amino_acid_changeQ/*
F01G10.8a.1VEP_consequencestop_gained
VEP_impactHIGH
HGVScF01G10.8a.1:c.574C>T
HGVSpCE29090:p.Gln192Ter
cDNA_position574
CDS_position574
Protein_position192
Exon_number4/6
Codon_changeCaa/Taa
Amino_acid_changeQ/*
Interactor (58)
GeneticsInterpolated_map_positionIV4.56441
Mapping_dataIn_2_point436
437
In_multi_point (12)
In_pos_neg_data957
965
2738
DescriptionPhenotypeWBPhenotype:0000012Paper_evidenceWBPaper00000502
WBPaper00028386
WBPaper00032073
Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkRecovered by selection for resistance to 1% SDS.Paper_evidenceWBPaper00000502
Curator_confirmedWBPerson712
Animals formed less than 10% dauer at 16C.Paper_evidenceWBPaper00028386
Curator_confirmedWBPerson712
Constitutive dauer formation at 25C, incomplete penetrance, reversible by shift to 15C. Easy to score (ES3) at L3.Person_evidenceWBPerson261
Curator_confirmedWBPerson712
PenetranceIncompletePerson_evidenceWBPerson261
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00000502
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00000502
WBPaper00028386
Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Phenotype_assayTemperature16Paper_evidenceWBPaper00028386
Curator_confirmedWBPerson712
25Paper_evidenceWBPaper00032073
Curator_confirmedWBPerson712
Genotypeunc-4(e120)Paper_evidenceWBPaper00028386
Curator_confirmedWBPerson712
Ease_of_scoringES3_Easy_to_scorePerson_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0000137Paper_evidenceWBPaper00038374
Curator_confirmedWBPerson2987
Remark"We tested changes in the levels of these insulins using Q-PCR in TGFb pathway mutants such as daf-3(mgDf90), daf-14(m77) as well as pdp-1(tm3734) and compared them to wild-type worms (Figure 5A-5C, Figure S14, Tables S2 and S3). Interestingly, both pdp-1(tm3734) and daf-3(mgDf90) showed elevated levels of several insulins as compared to wild-type worms (Figure 5A and Figure S14). In contrast, expression of these insulins was markedly reduced in daf-14(m77) mutants (Figure 5B and Figure S14)."Paper_evidenceWBPaper00038374
Curator_confirmedWBPerson2987
WBPhenotype:0000294Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkDark intestine.Person_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0000545Paper_evidenceWBPaper00000635
Curator_confirmedWBPerson48
RemarkLate-stage eggs are laid.Paper_evidenceWBPaper00000635
Curator_confirmedWBPerson48
WBPhenotype:0000640Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkType C Egl; dark intestine.Person_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0000660Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkclumpyPerson_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0001013Paper_evidenceWBPaper00032501
Curator_confirmedWBPerson2021
RemarkMutants had enhanced susceptibility to killing by PA14 than N2Paper_evidenceWBPaper00032501
Curator_confirmedWBPerson2021
WBPhenotype:0001411Person_evidenceWBPerson261
Curator_confirmedWBPerson712
RemarkSrf-6 at 16C.Person_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0001690Paper_evidenceWBPaper00002589
Curator_confirmedWBPerson712
RemarkAnimals bind mAb M37 at stages L1-L4 but not as adults, wildtype worms only bind this anti-body at the L1 stage. Animals in dauer stage do not bind mAb 37, similar to wildtype.Paper_evidenceWBPaper00002589
Curator_confirmedWBPerson712
PenetranceHighPentrance ranged from 87-100%.Paper_evidenceWBPaper00002589
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25CPaper_evidenceWBPaper00002589
Curator_confirmedWBPerson712
Phenotype_assayTemperature16Paper_evidenceWBPaper00002589
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0000039Paper_evidenceWBPaper00038374
Curator_confirmedWBPerson2987
Remark"In our hands, mutations in the upstream components of the TGF-b pathway such as daf-7 and daf-14 enhance dauer formation but do not significantly extend lifespan (Table S1 and Figure S4)."Paper_evidenceWBPaper00038374
Curator_confirmedWBPerson2987
Phenotype_assayGenotypedaf-2(e1370)Paper_evidenceWBPaper00038374
Curator_confirmedWBPerson2987
WBPhenotype:0000061Paper_evidenceWBPaper00001837
Curator_confirmedWBPerson712
RemarkLifespans of adults were not noticeable different from wild type. Lifespans were examined when grown continuously at 15 deg C, or when shifted from 15 to 20 deg C and from 15 to 25 deg C as L4 animals.Paper_evidenceWBPaper00001837
Curator_confirmedWBPerson712
WBPhenotype:0001033Paper_evidenceWBPaper00035610
Curator_confirmedWBPerson2021
RemarkNo extension of the mitotic zone.Paper_evidenceWBPaper00035610
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentDAPI stainingPaper_evidenceWBPaper00035610
Curator_confirmedWBPerson2021
WBPhenotype:0001068Paper_evidenceWBPaper00000635
Curator_confirmedWBPerson48
Phenotype_assayTreatment5 mg/ml serotoninPaper_evidenceWBPaper00000635
Curator_confirmedWBPerson48
WBPhenotype:0001215Paper_evidenceWBPaper00035610
Curator_confirmedWBPerson2021
RemarkThe number of PH3-positive cells in daf-14(m77) mutants was comparable to that in the wild-type germ linePaper_evidenceWBPaper00035610
Curator_confirmedWBPerson2021
Phenotype_assayTreatmentanti-phospho-histone H3 antibody stainingPaper_evidenceWBPaper00035610
Curator_confirmedWBPerson2021
WBPhenotype:0001340Paper_evidenceWBPaper00000635
Curator_confirmedWBPerson48
Phenotype_assayTreatment0.75 mg/ml imipraminePaper_evidenceWBPaper00000635
Curator_confirmedWBPerson48
WBPhenotype:0002535Paper_evidenceWBPaper00000932
Curator_confirmedWBPerson712
RemarkFITC uptake is normal.Paper_evidenceWBPaper00000932
Curator_confirmedWBPerson712
Reference (20)
MethodSubstitution_allele