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WormBase Tree Display for Variation: WBVar00089266

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Name Class

WBVar00089266EvidencePaper_evidenceWBPaper00031689
NamePublic_namemy15
Sequence_detailsSeqStatusPending_curation
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00031070
LaboratoryPT
StatusLive
AffectsGeneWBGene00086546
DescriptionPhenotypeWBPhenotype:0000387Paper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
RemarkIsolated my15 sperm are immotile.Paper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
WBPhenotype:0000436Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
RemarkClass A Cil. Animals exhibited abnormal distribution of PKD-2::GFP in the CEM dendrites of males. PDK-2::GFP was evenly distributed in the rays.Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0005246PATO:0000460Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
WBbt:0006941PATO:0000460Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
Life_stageWBls:0000056PATO:0000460Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
Phenotype_assayGenotypemyIs1[Ppkd-2::PKD-2::GFP;Pcc::GFP] pkd-2(sy606);him-5e1490).Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
WBPhenotype:0000509Paper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
RemarkWhen present, my15 pseudopods are significantly shorter than WT.Paper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
WBPhenotype:0000670Paper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
Remarkmy15 males produce few offspring because of a sperm (Spe) defectPaper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000056PATO:0000460Paper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
WBPhenotype:0000843Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
RemarkMating efficiency is significantly less than control levels.Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0005246PATO:0000460Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
Life_stageWBls:0000056PATO:0000460Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
Phenotype_assayTreatmentFor each trial, six males and two dpy-17(e904) hermaphrodites were placed on a plate.Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
Genotypehim-5(e1490)Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
WBPhenotype:0000863Paper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
Remarkmy15 males are largely infertilePaper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
EQ_annotationsLife_stageWBls:0000056PATO:0000460Paper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
WBPhenotype:0000882Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
RemarkClass A Cil. Animals exhibited abnormal distribution of PKD-2::GFP in the CEM dendrites of males.Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0005246PATO:0000460Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
Life_stageWBls:0000056PATO:0000460Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
Phenotype_assayGenotypemyIs1[Ppkd-2::PKD-2::GFP;Pcc::GFP] pkd-2(sy606);him-5e1490).Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
WBPhenotype:0001409Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
RemarkClass A Cil. Animals exhibited ectopic distribution of PKD-2::GFP.Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0005246PATO:0000460Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
Life_stageWBls:0000056PATO:0000460Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
Phenotype_assayGenotypemyIs1[Ppkd-2::PKD-2::GFP;Pcc::GFP] pkd-2(sy606);him-5e1490).Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
WBPhenotype:0002038Paper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
RemarkOf many GFP-tagged ciliary proteins, only LOV-1::GFP is abnormally distributed to dendritic and axonal processes in sensory neurons. Also, PKD-2::GFP is distributed throughout these male-specific neurons including dendrites, axons, cell bodies, and cilia, whereas in wild-type, it localizes to cell bodies and ciliary endings only.Paper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0000436Paper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
RemarkIn WT and my15 spermatides, anti-TRP-3 labels cytoplasmic puncta partially overlapping with MOs. In WT and my15 spermatozoa, TRP-3 is detected ubiquitously on the PM of the pseudopod and cell body.Paper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
WBPhenotype:0000637Paper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
Remarkmy15 mutants are normal in dauer formationPaper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
WBPhenotype:0000649Paper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
Remarkmy15 males exhibit normal response and vulva location behaviorsPaper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
WBPhenotype:0000663Paper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
Remarkmy15 mutants are normal in osmotic avoidance (data not shown).Paper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
WBPhenotype:0000847Paper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
RemarkSNP-1::GFP labels presynaptic puncta along axonal processesPaper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
WBPhenotype:0001344Paper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
RemarkIn my15 sperm, 1CB4 staining for MO morphology and localization before and after activation appears to be WT. | in both WT and my15, FMI-43 labels the PM of the round spermatids and, after activation, concentrates at the site of membrane fusion on the spermatozoon cell body.Paper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
WBPhenotype:0001470Paper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
Remarkmy15 mutants are normal in chemotaxis to diacetylPaper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00002819Paper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
WBPhenotype:0001510Paper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
RemarkNeurons express cell-specific reporters.Paper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
WBPhenotype:0002535Paper_evidenceWBPaper00031689
WBPaper00035231
Curator_confirmedWBPerson712
RemarkAnimals do not exhibit DiI filling defects.Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
my15 mutants are normal in lipophilic DiI dye filling of ciliated sensory neuronsPaper_evidenceWBPaper00035231
Curator_confirmedWBPerson712
WBPhenotype:0004004Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
RemarkMale response efficiency was not significantly different from control animals.Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0005246PATO:0000460Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
Life_stageWBls:0000056PATO:0000460Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
Phenotype_assayTreatmentEach trial consists of 10-25 males. Males were assayed 4 minutes after contact.Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
GenotypemyIs1[Ppkd-2::PKD-2::GFP;Pcc::GFP] pkd-2(sy606);him-5e1490).Paper_evidenceWBPaper00031689
Curator_confirmedWBPerson712
ReferenceWBPaper00031689
WBPaper00035231
MethodAllele