WormBase Tree Display for Variation: WBVar00089266
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WBVar00089266 | Evidence | Paper_evidence | WBPaper00031689 | ||||||
---|---|---|---|---|---|---|---|---|---|
Name | Public_name | my15 | |||||||
Sequence_details | SeqStatus | Pending_curation | |||||||
Variation_type | Allele | ||||||||
Origin | Species | Caenorhabditis elegans | |||||||
Strain | WBStrain00031070 | ||||||||
Laboratory | PT | ||||||||
Status | Live | ||||||||
Affects | Gene | WBGene00086546 | |||||||
Description | Phenotype | WBPhenotype:0000387 | Paper_evidence | WBPaper00035231 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Isolated my15 sperm are immotile. | Paper_evidence | WBPaper00035231 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000436 | Paper_evidence | WBPaper00031689 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Class A Cil. Animals exhibited abnormal distribution of PKD-2::GFP in the CEM dendrites of males. PDK-2::GFP was evenly distributed in the rays. | Paper_evidence | WBPaper00031689 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0005246 | PATO:0000460 | Paper_evidence | WBPaper00031689 | ||||
Curator_confirmed | WBPerson712 | ||||||||
WBbt:0006941 | PATO:0000460 | Paper_evidence | WBPaper00031689 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Life_stage | WBls:0000056 | PATO:0000460 | Paper_evidence | WBPaper00031689 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Genotype | myIs1[Ppkd-2::PKD-2::GFP;Pcc::GFP] pkd-2(sy606);him-5e1490). | Paper_evidence | WBPaper00031689 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000509 | Paper_evidence | WBPaper00035231 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | When present, my15 pseudopods are significantly shorter than WT. | Paper_evidence | WBPaper00035231 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000670 | Paper_evidence | WBPaper00035231 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | my15 males produce few offspring because of a sperm (Spe) defect | Paper_evidence | WBPaper00035231 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Life_stage | WBls:0000056 | PATO:0000460 | Paper_evidence | WBPaper00035231 | ||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000843 | Paper_evidence | WBPaper00031689 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Mating efficiency is significantly less than control levels. | Paper_evidence | WBPaper00031689 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0005246 | PATO:0000460 | Paper_evidence | WBPaper00031689 | ||||
Curator_confirmed | WBPerson712 | ||||||||
Life_stage | WBls:0000056 | PATO:0000460 | Paper_evidence | WBPaper00031689 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Treatment | For each trial, six males and two dpy-17(e904) hermaphrodites were placed on a plate. | Paper_evidence | WBPaper00031689 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Genotype | him-5(e1490) | Paper_evidence | WBPaper00031689 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000863 | Paper_evidence | WBPaper00035231 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | my15 males are largely infertile | Paper_evidence | WBPaper00035231 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Life_stage | WBls:0000056 | PATO:0000460 | Paper_evidence | WBPaper00035231 | ||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000882 | Paper_evidence | WBPaper00031689 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Class A Cil. Animals exhibited abnormal distribution of PKD-2::GFP in the CEM dendrites of males. | Paper_evidence | WBPaper00031689 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0005246 | PATO:0000460 | Paper_evidence | WBPaper00031689 | ||||
Curator_confirmed | WBPerson712 | ||||||||
Life_stage | WBls:0000056 | PATO:0000460 | Paper_evidence | WBPaper00031689 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Genotype | myIs1[Ppkd-2::PKD-2::GFP;Pcc::GFP] pkd-2(sy606);him-5e1490). | Paper_evidence | WBPaper00031689 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001409 | Paper_evidence | WBPaper00031689 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Class A Cil. Animals exhibited ectopic distribution of PKD-2::GFP. | Paper_evidence | WBPaper00031689 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0005246 | PATO:0000460 | Paper_evidence | WBPaper00031689 | ||||
Curator_confirmed | WBPerson712 | ||||||||
Life_stage | WBls:0000056 | PATO:0000460 | Paper_evidence | WBPaper00031689 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Genotype | myIs1[Ppkd-2::PKD-2::GFP;Pcc::GFP] pkd-2(sy606);him-5e1490). | Paper_evidence | WBPaper00031689 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0002038 | Paper_evidence | WBPaper00035231 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Of many GFP-tagged ciliary proteins, only LOV-1::GFP is abnormally distributed to dendritic and axonal processes in sensory neurons. Also, PKD-2::GFP is distributed throughout these male-specific neurons including dendrites, axons, cell bodies, and cilia, whereas in wild-type, it localizes to cell bodies and ciliary endings only. | Paper_evidence | WBPaper00035231 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_not_observed | WBPhenotype:0000436 | Paper_evidence | WBPaper00035231 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | In WT and my15 spermatides, anti-TRP-3 labels cytoplasmic puncta partially overlapping with MOs. In WT and my15 spermatozoa, TRP-3 is detected ubiquitously on the PM of the pseudopod and cell body. | Paper_evidence | WBPaper00035231 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000637 | Paper_evidence | WBPaper00035231 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | my15 mutants are normal in dauer formation | Paper_evidence | WBPaper00035231 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000649 | Paper_evidence | WBPaper00035231 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | my15 males exhibit normal response and vulva location behaviors | Paper_evidence | WBPaper00035231 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000663 | Paper_evidence | WBPaper00035231 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | my15 mutants are normal in osmotic avoidance (data not shown). | Paper_evidence | WBPaper00035231 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0000847 | Paper_evidence | WBPaper00035231 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | SNP-1::GFP labels presynaptic puncta along axonal processes | Paper_evidence | WBPaper00035231 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001344 | Paper_evidence | WBPaper00035231 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | In my15 sperm, 1CB4 staining for MO morphology and localization before and after activation appears to be WT. | in both WT and my15, FMI-43 labels the PM of the round spermatids and, after activation, concentrates at the site of membrane fusion on the spermatozoon cell body. | Paper_evidence | WBPaper00035231 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001470 | Paper_evidence | WBPaper00035231 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | my15 mutants are normal in chemotaxis to diacetyl | Paper_evidence | WBPaper00035231 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Affected_by | Molecule | WBMol:00002819 | Paper_evidence | WBPaper00035231 | |||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0001510 | Paper_evidence | WBPaper00035231 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Neurons express cell-specific reporters. | Paper_evidence | WBPaper00035231 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0002535 | Paper_evidence | WBPaper00031689 | |||||||
WBPaper00035231 | |||||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Animals do not exhibit DiI filling defects. | Paper_evidence | WBPaper00031689 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
my15 mutants are normal in lipophilic DiI dye filling of ciliated sensory neurons | Paper_evidence | WBPaper00035231 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
WBPhenotype:0004004 | Paper_evidence | WBPaper00031689 | |||||||
Curator_confirmed | WBPerson712 | ||||||||
Remark | Male response efficiency was not significantly different from control animals. | Paper_evidence | WBPaper00031689 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
EQ_annotations | Anatomy_term | WBbt:0005246 | PATO:0000460 | Paper_evidence | WBPaper00031689 | ||||
Curator_confirmed | WBPerson712 | ||||||||
Life_stage | WBls:0000056 | PATO:0000460 | Paper_evidence | WBPaper00031689 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Phenotype_assay | Treatment | Each trial consists of 10-25 males. Males were assayed 4 minutes after contact. | Paper_evidence | WBPaper00031689 | |||||
Curator_confirmed | WBPerson712 | ||||||||
Genotype | myIs1[Ppkd-2::PKD-2::GFP;Pcc::GFP] pkd-2(sy606);him-5e1490). | Paper_evidence | WBPaper00031689 | ||||||
Curator_confirmed | WBPerson712 | ||||||||
Reference | WBPaper00031689 | ||||||||
WBPaper00035231 | |||||||||
Method | Allele |