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WormBase Tree Display for Variation: WBVar00089664

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Name Class

WBVar00089664EvidencePaper_evidenceWBPaper00001778
NamePublic_namen676n909
Sequence_detailsSeqStatusPending_curation
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
LaboratoryMT
StatusLive
Linked_toWBVar00089663
WBVar00089818
AffectsGeneWBGene00003001
InteractorWBInteraction000517302
WBInteraction000517304
WBInteraction000519826
WBInteraction000541803
DescriptionPhenotypeWBPhenotype:0000594Paper_evidenceWBPaper00004662
Curator_confirmedWBPerson2987
Remark"We studied P11/12 in lin-12 mutants (glp-1 mutants die as young L1 larvae before P cell migration). P11 and P12 fates are not affected in any of the mutants (or only slightly for lin-12(n676n930); Table 1C and Greenwald et_al, 1983). In contrast, P(11/ 12)L/R migration is clearly affected and apparently randomized in three lin-12 reduction-of-function alleles, n137n720 (Seydoux et_al, 1990), n676n930 (Sundaram and Greenwald, 1993), and n676n909 (Seydoux et_al, 1990), and in the putative null allele n941 (Wen and Greenwald, 1999) (Table 1C)."Paper_evidenceWBPaper00004662
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0004409PATO:0000460Paper_evidenceWBPaper00004662
Curator_confirmedWBPerson2987
WBPhenotype:0000697Paper_evidenceWBPaper00001778
Curator_confirmedWBPerson712
RemarkAnimals display a single large protrusion at the site of the normal vulval position.Paper_evidenceWBPaper00001778
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00001778
Curator_confirmedWBPerson712
Variation_effectLoss_of_function_undetermined_extentPaper_evidenceWBPaper00001778
Curator_confirmedWBPerson712
WBPhenotype:0000699Person_evidenceWBPerson261
Curator_confirmedWBPerson712
Remarkintragenic revertant of dominant allele, resembles n941 lf phenotypePerson_evidenceWBPerson261
Curator_confirmedWBPerson712
WBPhenotype:0000961Paper_evidenceWBPaper00004481
Curator_confirmedWBPerson2987
Remark"We then examined patterning of isolated primary lineages with multiple ACs: we ablated P5.p and P7.p, but left the multiple ACs intact. The ACs attached to the two inner P6.pxx cells in 12 of 14 animals, and the wild-type zmp-1::GFP expression pattern in P6.pxxx cells was observed (Table 3B, Fig 3A,B). The ACs in the remaining two animals attached to one of the two outer P6.pxx cells, in addition to the two inner P6.pxx cells. Subsequently, the inner P6.pxxx cells, as well as the P6.pxxx progeny of the outer P6.pxx attached to the ACs, failed to express zmp-1::GFP, while the P6.pxxx progeny of the other outer P6.pxx expressed GFP (Table 3B, Fig 3C,D). Therefore, the AC can signal the P6.pxx cells that it contacts to generate vulF progeny."Paper_evidenceWBPaper00004481
Curator_confirmedWBPerson2987
"To examine the patterning of two adjacent primary lineages, we ablated P7.p in a lin-12(lf) background. When we left multiple ACs intact, the ACs attached to various numbers of posterior P5.pxxx cells and anterior P6.pxxx cells (data not shown). Subsequently, 137 of 144 central Pn.pxxx cells (P5.ppax, P5.pppx, P6.paax and P6.papx) behaved as vulF and did not express the marker (e.g. Fig 3E,F). When we ablated all ACs at early L3 before VPCs' first division, only 22 of 48 central Pn.pxxx cells did not express the marker (P < 0.0001). We conclude that the AC may signal the P6.pxx cells that it contacts to ensure that vulF progeny will be generated."Paper_evidenceWBPaper00004481
Curator_confirmedWBPerson2987
"To determine whether there are any other intercellular signaling mechanisms involved in 1&deg; lineage patterning besides AC signaling, we ablated P7.p and all but one AC in a lin-12(lf) background to examine patterning of adjacent 1&deg; lineages with a single AC. In all 14 animals examined, the Pn.pxx cells that attached to the AC gave rise to progeny that did not express GFP (as vulF), while their flanking Pn.pxx cells always produced progeny that expressed GFP (as vulE) (Table 3C, Fig 3G,H). The rest of the Pn.pxxx cells showed a random pattern of GFP expression. Therefore, the AC signals the 1&deg; VPC granddaughters it contacts to produce vulF progeny. Their immediate neighbors invariably generate vulE progeny. However, the progeny of the more distal neighbors do not form an invariant pattern. Notably, the Pn.pxxx cells surrounding the AC that form the E-F-F-E pattern do not always descend from the same 1&deg; VPC daughter and therefore do not always possess internal differences through intrinsically polar mother cells. We infer that in addition to AC signaling, there is likely signaling between the inner and outer 1&deg; VPC descendants (P6.pxx or P6.pxxx cells) in patterning the 1&deg; lineage."Paper_evidenceWBPaper00004481
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0007809PATO:0000460Paper_evidenceWBPaper00004481
Curator_confirmedWBPerson2987
Phenotype_assayGenotypesyIs49 [zmp-1::GFP]Paper_evidenceWBPaper00004481
Curator_confirmedWBPerson2987
Phenotype_not_observedWBPhenotype:0000239Paper_evidenceWBPaper00004481
Curator_confirmedWBPerson2987
Remark"Also, in lin-12(lf) mutants, where the &pi; fate is not specified, isolated 1&deg; lineages can invariably form a correct pattern (Newman et al., 1995; Table 3A; also see below)."Paper_evidenceWBPaper00004481
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0007809PATO:0000460Paper_evidenceWBPaper00004481
Curator_confirmedWBPerson2987
Phenotype_assayGenotypesyIs49 [zmp-1::GFP]Paper_evidenceWBPaper00004481
Curator_confirmedWBPerson2987
WBPhenotype:0000414Paper_evidenceWBPaper00004662
Curator_confirmedWBPerson2987
Remark"We studied P11/12 in lin-12 mutants (glp-1 mutants die as young L1 larvae before P cell migration). P11 and P12 fates are not affected in any of the mutants (or only slightly for lin-12(n676n930); Table 1C and Greenwald et_al, 1983)."Paper_evidenceWBPaper00004662
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0004409PATO:0000460Paper_evidenceWBPaper00004662
Curator_confirmedWBPerson2987
WBPhenotype:0000961Paper_evidenceWBPaper00004481
Curator_confirmedWBPerson2987
Remark"In addition, in lin-12(lf) mutants where the secondary fate is not specified, isolated primary lineages invariably had the wild-type expression pattern of zmp-1::GFP (Greenwald et al., 1983; Table 3A; also see below)."Paper_evidenceWBPaper00004481
Curator_confirmedWBPerson2987
"We first ablated P5.p and P7.p, as well as all but one AC. In all six animals scored, the AC attached to the two inner P6.pxx cells during L3 lethargus. zmp-1::GFP was expressed in the outer P6.pxxx cells (as vulE), but not the inner cells (as vulF), during L4 lethargus (Table 3A). This indicates that a single primary lineage with a single AC in a lin-12(lf) background patterns correctly, and that lin-12 is not required for proper patterning of the primary lineage."Paper_evidenceWBPaper00004481
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0007809PATO:0000460Paper_evidenceWBPaper00004481
Curator_confirmedWBPerson2987
Phenotype_assayGenotypesyIs49 [zmp-1::GFP]Paper_evidenceWBPaper00004481
Curator_confirmedWBPerson2987
ReferenceWBPaper00001778
WBPaper00004481
WBPaper00004662
RemarkCis double mutant
MethodAllele