Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Variation: WBVar00089684

expand all nodes | collapse all nodes | view schema

Name Class

WBVar00089684EvidencePaper_evidenceWBPaper00002543
NamePublic_namen698
Other_nameY71F9B.5b.1:c.66+1G>A
Y71F9B.5a.1:c.66+1G>A
Y71F9B.5a.2:c.66+1G>A
HGVSgCHROMOSOME_I:g.2707695G>A
Sequence_detailsSMapS_parentSequenceY71F9B
Flanking_sequencesccactcgccacaggatcaattttcgatcagtagacttattggaaatctgaaaaatcgatg
Mapping_targetY71F9B
Type_of_mutationSubstitutiongaPaper_evidenceWBPaper00002543
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00026852
LaboratoryMT
StatusLive
AffectsGeneWBGene00003006
TranscriptY71F9B.5a.1VEP_consequencesplice_donor_variant
VEP_impactHIGH
HGVScY71F9B.5a.1:c.66+1G>A
Intron_number2/10
Y71F9B.5b.1VEP_consequencesplice_donor_variant
VEP_impactHIGH
HGVScY71F9B.5b.1:c.66+1G>A
Intron_number2/10
Y71F9B.5a.2VEP_consequencesplice_donor_variant
VEP_impactHIGH
HGVScY71F9B.5a.2:c.66+1G>A
Intron_number3/11
InteractorWBInteraction000000820
WBInteraction000524002
WBInteraction000524003
WBInteraction000524042
WBInteraction000538570
WBInteraction000538577
GeneticsInterpolated_map_positionI-7.44605
DescriptionPhenotypeWBPhenotype:0000257Paper_evidenceWBPaper00002543
Curator_confirmedWBPerson712
RemarkAdult hermaphrodites exhibited phasmid defects as assayed by DiO dye-filling.Paper_evidenceWBPaper00002543
Curator_confirmedWBPerson712
WBPhenotype:0000414Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
Remarklin-17(n698) confers a SPD sheath to neuron fate transformation (Table 1)Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0005831PATO:0000460Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
WBPhenotype:0000691Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
Remark8% of lin-17(n698) hermaphrodite animals have a gonad defect (defined as abnormal gonad morphology, single gonadal arm, and ectopic meiosis at L4 stage) (Table 2).Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
PenetranceLowPaper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0005175PATO:0000460Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
WBPhenotype:0000700Paper_evidenceWBPaper00000762
Curator_confirmedWBPerson2021
RemarkSingle protrusion posterior to the vulvaPaper_evidenceWBPaper00000762
Curator_confirmedWBPerson2021
PenetranceLowPaper_evidenceWBPaper00000762
Curator_confirmedWBPerson2021
RecessivePaper_evidenceWBPaper00000762
Curator_confirmedWBPerson2021
EQ_annotationsLife_stageWBls:0000057PATO:0000460Paper_evidenceWBPaper00000762
Curator_confirmedWBPerson2021
WBPhenotype:0000828Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
Remark73% of lin-17(n698) animals have abnormal phasmids, as determined by DiO filling assay, indicative of a T-lineage defect.Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
PenetranceIncompletePaper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0005425PATO:0000460Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
Phenotype_assayTreatmentL3 and L4 stage worms were tested by DiO filling of amphids and phasmids, as described by Herman and Horvitz (1994). Animals that showed positive for amphid filling and negative for phasmid filling were scored as phasmid defective.Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
WBPhenotype:0001276Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
RemarkUsing a weak lin-17 allele, n698, wherein the first B cell division is usually not disrupted, we found that 80% of the animals show two cells per spicule (one neuron, one sheath) expressing the SPD marker gpa-1::lacZ (Fig. 2D; Table 1), as opposed to just one cell (SPD neuron) in wild type animalsPaper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
In the lin-17(n698) mutant, osm-6::GFP is expressed in an extra cell associated with the spicules (four of six animals examined), consistent with the extra expression observed with the gpa-1::lacZ marker.Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0005831PATO:0000460Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
Phenotype_assayGenotypesyIs20[gpa-1::lacZ+dpy-20(+)] (marker for male spicules and phasmid neurons)Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
osm-6::GFP (marker expressed in SPD and SPV neurons of wild type spicules and in male sensory ray neurons)Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
WBPhenotype:0001355Paper_evidenceWBPaper00005832
Curator_confirmedWBPerson2987
RemarkTable 2Paper_evidenceWBPaper00005832
Curator_confirmedWBPerson2987
PenetranceLow6Paper_evidenceWBPaper00005832
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0005175PATO:0000460Paper_evidenceWBPaper00005832
Curator_confirmedWBPerson2987
WBPhenotype:0001681Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
Remarkabnormal spindle orientation of BγPaper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
EQ_annotationsAnatomy_termWBbt:0007830PATO:0000460Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
GO_termGO:0000132PATO:0000460Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
GO:0005819PATO:0000460Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
WBPhenotype:0002011Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
Remark2% of lin-17(n698) animals have a bivulva phenotype (Table 2)Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
PenetranceLowPaper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0006748PATO:0000460Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
Phenotype_not_observedWBPhenotype:0000099Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
Remarklin-17(n698) animals did not exhibit a P12 fate specification defectPaper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0004409PATO:0000460Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
WBPhenotype:0000306Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
RemarkNo alteration in syIs45 expressionPaper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
EQ_annotationsAnatomy_termWBbt:0007830PATO:0000460Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
WBbt:0008451PATO:0000460Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
GO_termGO:0010467PATO:0000460Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
Phenotype_assayControl_strainWBStrain00044748Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
Treatmentceh-13::gfp as marker of Bγ cell fate syIs145 PS4807 contains the ceh-13::GFP integrated transgene syIs145 that was obtained by microinjection of pMF1 at 10 ng/μl, pBS at 20 ng/μl and unc-119(+) at 40 ng/μl into unc-119(ed4); him-5(e1490) mutant animals.Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
GenotypesIys145 [ceh-13::GFP]Paper_evidenceWBPaper00035553
Curator_confirmedWBPerson625
WBPhenotype:0000961Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
RemarkExpression of a socket cell marker, egl-17::GFP, was not affected by the lin-17(n698) mutation (n = 10), suggesting that the socket cells are correctly specified in lin-17(n698) mutants.Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0005750PATO:0000460Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
Phenotype_assayGenotypeegl-17::GFP (socket cell marker)Paper_evidenceWBPaper00003453
Curator_confirmedWBPerson2987
ReferenceWBPaper00003453
WBPaper00035553
WBPaper00000762
WBPaper00005832
WBPaper00002543
WBPaper00017527
MethodSubstitution_allele