WormBase Tree Display for Variation: WBVar00092136

WBVar00092136 Name: Public_name: ok861
    Other_name: R07E5.8a.2:c.599+46_1376delinsTCGATCGATGTCTGCTGCGCACTTAGCATAAGGTTTCCCAAAATTTCGTTGCTCTGATCAAAGATCAAACTACATAGGGATAGAAGGACACGCGCTTTATATAGTAAGTGATTTTTAAATCAATTTTAGAATTTCAGCAATTTTCTTTCGGTTTCACAAGTTTTTTTTGGAAAATAGTCTGAAAATTCGACAAACGATGTTTCAAAGAAAAACTAATGAAACTGTTGTGCAAAATTTTAGATTTTTTAGGAAAACTTAATAATTTTTCAAACGTAAATTCGTAATTACAGTTCTGAGATTTTTGCAGTTAATTTC
      R07E5.8a.1:c.599+46_1376delinsTCGATCGATGTCTGCTGCGCACTTAGCATAAGGTTTCCCAAAATTTCGTTGCTCTGATCAAAGATCAAACTACATAGGGATAGAAGGACACGCGCTTTATATAGTAAGTGATTTTTAAATCAATTTTAGAATTTCAGCAATTTTCTTTCGGTTTCACAAGTTTTTTTTGGAAAATAGTCTGAAAATTCGACAAACGATGTTTCAAAGAAAAACTAATGAAACTGTTGTGCAAAATTTTAGATTTTTTAGGAAAACTTAATAATTTTTCAAACGTAAATTCGTAATTACAGTTCTGAGATTTTTGCAGTTAATTTC
    HGVSg: CHROMOSOME_III:g.4420642_4422287delinsTCGATCGATGTCTGCTGCGCACTTAGCATAAGGTTTCCCAAAATTTCGTTGCTCTGATCAAAGATCAAACTACATAGGGATAGAAGGACACGCGCTTTATATAGTAAGTGATTTTTAAATCAATTTTAGAATTTCAGCAATTTTCTTTCGGTTTCACAAGTTTTTTTTGGAAAATAGTCTGAAAATTCGACAAACGATGTTTCAAAGAAAAACTAATGAAACTGTTGTGCAAAATTTTAGATTTTTTAGGAAAACTTAATAATTTTTCAAACGTAAATTCGTAATTACAGTTCTGAGATTTTTGCAGTTAATTTC
  Sequence_details: SMap: S_parent: Sequence: R07E5
    Flanking_sequences: taaaattggcgatttttgcgccaaaaatac tgaccttatcgattgtatgcaattacaaga
    Mapping_target: R07E5
    Type_of_mutation: Insertion: TCGATCGATGTCTGCTGCGCACTTAGCATAAGGTTTCCCAAAATTTCGTTGCTCTGATCAAAGATCAAACTACATAGGGATAGAAGGACACGCGCTTTATATAGTAAGTGATTTTTAAATCAATTTTAGAATTTCAGCAATTTTCTTTCGGTTTCACAAGTTTTTTTTGGAAAATAGTCTGAAAATTCGACAAACGATGTTTCAAAGAAAAACTAATGAAACTGTTGTGCAAAATTTTAGATTTTTTAGGAAAACTTAATAATTTTTCAAACGTAAATTCGTAATTACAGTTCTGAGATTTTTGCAGTTAATTTC
      Deletion
    PCR_product: ok861_external
      ok861_internal
    SeqStatus: Sequenced
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00022650
      WBStrain00031674
    Laboratory: RB
    Person: WBPerson46
    KO_consortium_allele
    Status: Live
  Affects: Gene: WBGene00000520
    Transcript: R07E5.8a.2 VEP_consequence: splice_acceptor_variant,splice_donor_variant,coding_sequence_variant,intron_variant
        VEP_impact: HIGH
        HGVSc: R07E5.8a.2:c.599+46_1376delinsTCGATCGATGTCTGCTGCGCACTTAGCATAAGGTTTCCCAAAATTTCGTTGCTCTGATCAAAGATCAAACTACATAGGGATAGAAGGACACGCGCTTTATATAGTAAGTGATTTTTAAATCAATTTTAGAATTTCAGCAATTTTCTTTCGGTTTCACAAGTTTTTTTTGGAAAATAGTCTGAAAATTCGACAAACGATGTTTCAAAGAAAAACTAATGAAACTGTTGTGCAAAATTTTAGATTTTTTAGGAAAACTTAATAATTTTTCAAACGTAAATTCGTAATTACAGTTCTGAGATTTTTGCAGTTAATTTC
        cDNA_position: ?-1378
        CDS_position: ?-1376
        Protein_position: ?-459
        Intron_number: 5-9/14
        Exon_number: 6-10/15
      R07E5.8a.1 VEP_consequence: splice_acceptor_variant,splice_donor_variant,coding_sequence_variant,intron_variant
        VEP_impact: HIGH
        HGVSc: R07E5.8a.1:c.599+46_1376delinsTCGATCGATGTCTGCTGCGCACTTAGCATAAGGTTTCCCAAAATTTCGTTGCTCTGATCAAAGATCAAACTACATAGGGATAGAAGGACACGCGCTTTATATAGTAAGTGATTTTTAAATCAATTTTAGAATTTCAGCAATTTTCTTTCGGTTTCACAAGTTTTTTTTGGAAAATAGTCTGAAAATTCGACAAACGATGTTTCAAAGAAAAACTAATGAAACTGTTGTGCAAAATTTTAGATTTTTTAGGAAAACTTAATAATTTTTCAAACGTAAATTCGTAATTACAGTTCTGAGATTTTTGCAGTTAATTTC
        cDNA_position: ?-1384
        CDS_position: ?-1376
        Protein_position: ?-459
        Intron_number: 5-9/14
        Exon_number: 6-10/15
      R07E5.8b.1 VEP_consequence: splice_acceptor_variant,splice_donor_variant,coding_sequence_variant,5_prime_UTR_variant,intron_variant
        VEP_impact: HIGH
        cDNA_position: ?-233
        CDS_position: ?-233
        Protein_position: ?-78
        Intron_number: 1/5
        Exon_number: 1-2/6
  Isolation: Mutagen: UV/TMP
  Description: Phenotype: WBPhenotype:0001921 Paper_evidence: WBPaper00044981
        Curator_confirmed: WBPerson3353
    Phenotype_not_observed: WBPhenotype:0001760 Paper_evidence: WBPaper00031945
        Curator_confirmed: WBPerson712
        Remark: MonoG or G-tracts were not deleted at an increased rate as demonstrated by lack of expression of B-galactosidase, which acted as an indicator of a DNA rearrangement bringing a LacZ start codon in-frame with the downstream ORF. No increase in deletion frequency over wild-type was observed for the endogenous qua375 sequence as determined by PCR. At least 24 populations of 5 animals were assayed. Paper_evidence: WBPaper00031945
            Curator_confirmed: WBPerson712
        Phenotype_assay: Genotype: pkIs2165[pRP1878: hsp-16.41::ATG-(C)23-stops-LacZ unc-119] or pkIs2172 [pRP1889: hsp-16.41::ATG-(monoA)-stops-LacZ unc-119] Paper_evidence: WBPaper00031945
              Curator_confirmed: WBPerson712
  Reference: WBPaper00031945
    WBPaper00025420
    WBPaper00044981
    WBPaper00064961
  Remark: Sequenced by the C. elegans Gene Knockout Consortium Paper_evidence: WBPaper00041807
  Method: KO_consortium_allele