Questions, Feedback & Help
Send us an email and we'll get back to you ASAP. Or you can read our Frequently Asked Questions.

WormBase Tree Display for Variation: WBVar00093027

expand all nodes | collapse all nodes | view schema

Name Class

WBVar00093027NamePublic_nameok1829
Other_nameF55A3.7:n.445_1276+179delinsTT
HGVSgCHROMOSOME_I:g.10785807_10787148delinsAA
Sequence_detailsSMapS_parentSequenceF55A3
Flanking_sequencestagattatctcgtgttaattcgcgaatataaactgcgaacagatgagtaaacgcgagagt
Mapping_targetF55A3
Type_of_mutationInsertionAA
Deletion
PCR_productOK1829_external
OK1829_internal
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00032220
WBStrain00048376
WBStrain00048380
LaboratoryRB
PersonWBPerson46
KO_consortium_allele
StatusLive
AffectsGeneWBGene00018852
PseudogeneF55A3.7VEP_consequencesplice_acceptor_variant,splice_donor_variant,non_coding_transcript_exon_variant,intron_variant
VEP_impactHIGH
HGVScF55A3.7:n.445_1276+179delinsTT
cDNA_position445-?
Intron_number2-3/3
Exon_number2-3/4
IsolationMutagenUV/TMP
DescriptionPhenotypeWBPhenotype:0000414Paper_evidenceWBPaper00061371
Curator_confirmedWBPerson712
RemarkIndeed, after broad induction of the ASE neuron specifying TF CHE-1 in L4 animals, ectopic expression of the ASE neuron fate reporter gcy-5p::GFP could be detected in the germline (assessed after 24 h) (Figure 1B). Some reprogrammed germ cells showed axo-dendritic projections (Figure 1B, bottom right), indicating that they acquired neuronal-like features.Paper_evidenceWBPaper00061371
Curator_confirmedWBPerson712
Phenotype_assayGenotypeBAT372 F55A3.7(ok1829) I; otIs305 [hsp-16.2p::che-1::3xHA, rol-6(su1006)], ntIs1 [gcy-5p::GFP, lin-15(+)] V OR BAT2192 F55A3.7(ok1829) I; otIs355 [rab-3::NLS::TagRFP]; otIs305 [hsp-16.2p::che-1::3xHA, rol-6(su1006)], ntIs1 [gcy-5p::GFP, lin-15(+)] V.Paper_evidenceWBPaper00061371
Curator_confirmedWBPerson712
WBPhenotype:0001276Paper_evidenceWBPaper00061371
Curator_confirmedWBPerson712
RemarkIndeed, after broad induction of the ASE neuron specifying TF CHE-1 in L4 animals, ectopic expression of the ASE neuron fate reporter gcy-5p::GFP could be detected in the germline (assessed after 24 h) (Figure 1B). Some reprogrammed germ cells showed axo-dendritic projections (Figure 1B, bottom right), indicating that they acquired neuronal-like features. Additionally, reprogrammed germ cells also express the pan-neuronal marker rab-3p::NLS::tagRFP, corroborating the notion that the sspt-16 depletion background allows conversion of germ cells to neuron like-cells upon induction of CHE-1 overexpression.Paper_evidenceWBPaper00061371
Curator_confirmedWBPerson712
Phenotype_assayGenotypeBAT372 F55A3.7(ok1829) I; otIs305 [hsp-16.2p::che-1::3xHA, rol-6(su1006)], ntIs1 [gcy-5p::GFP, lin-15(+)] V OR BAT2192 F55A3.7(ok1829) I; otIs355 [rab-3::NLS::TagRFP]; otIs305 [hsp-16.2p::che-1::3xHA, rol-6(su1006)], ntIs1 [gcy-5p::GFP, lin-15(+)] V.Paper_evidenceWBPaper00061371
Curator_confirmedWBPerson712
WBPhenotype:0001510Paper_evidenceWBPaper00061371
Curator_confirmedWBPerson712
RemarkIndeed, after broad induction of the ASE neuron specifying TF CHE-1 in L4 animals, ectopic expression of the ASE neuron fate reporter gcy-5p::GFP could be detected in the germline (assessed after 24 h) (Figure 1B). Some reprogrammed germ cells showed axo-dendritic projections (Figure 1B, bottom right), indicating that they acquired neuronal-like features.Paper_evidenceWBPaper00061371
Curator_confirmedWBPerson712
Phenotype_assayGenotypeBAT372 F55A3.7(ok1829) I; otIs305 [hsp-16.2p::che-1::3xHA, rol-6(su1006)], ntIs1 [gcy-5p::GFP, lin-15(+)] V OR BAT2192 F55A3.7(ok1829) I; otIs355 [rab-3::NLS::TagRFP]; otIs305 [hsp-16.2p::che-1::3xHA, rol-6(su1006)], ntIs1 [gcy-5p::GFP, lin-15(+)] V.Paper_evidenceWBPaper00061371
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0000717Paper_evidenceWBPaper00061371
Curator_confirmedWBPerson712
RemarkmRNA or protein levels analyzed through qPCR and whole worm western blots of FACT members, spt-16, hmg-3 and lin-53 did not differ from WT.Paper_evidenceWBPaper00061371
Curator_confirmedWBPerson712
Phenotype_assayGenotypegcy-5p::GFPPaper_evidenceWBPaper00061371
Curator_confirmedWBPerson712
WBPhenotype:0002632Paper_evidenceWBPaper00061371
Curator_confirmedWBPerson712
RemarkWe performed a germline-specific Assay for Transposase Accessible Chromatin with high-throughput sequencing (ATAC-seq). However, we found no significant differences in chromatin accessibility of WT vs. sspt-16(ok1829) germlines (Figure 1G).Paper_evidenceWBPaper00061371
Curator_confirmedWBPerson712
EQ_annotationsGO_termGO:0034401PATO:0000460Paper_evidenceWBPaper00061371
Curator_confirmedWBPerson712
Phenotype_assayGenotypegcy-5p::GFPPaper_evidenceWBPaper00061371
Curator_confirmedWBPerson712
ReferenceWBPaper00061371
RemarkSequenced by the C. elegans Gene Knockout ConsortiumPaper_evidenceWBPaper00041807
MethodKO_consortium_allele