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WormBase Tree Display for Variation: WBVar00093403

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Name Class

WBVar00093403NamePublic_nameok2239
HGVSgCHROMOSOME_III:g.7362292_7363266del
Sequence_detailsSMapS_parentSequenceF08F8
Flanking_sequencescgtaaatgaaaacaaattttgatggagattctgagacattgaaatcaaatttcctttgtc
Mapping_targetF08F8
Type_of_mutationDeletion
PCR_productok2239_external
ok2239_internal
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00032440
LaboratoryRB
PersonWBPerson46
KO_consortium_allele
StatusLive
AffectsGeneWBGene00017270
TranscriptF08F8.5.1VEP_consequencecoding_sequence_variant,3_prime_UTR_variant
VEP_impactMODIFIER
cDNA_position199-?
CDS_position199-?
Protein_position67-?
Exon_number1/1
IsolationMutagenEMS
DescriptionPhenotypeWBPhenotype:0000007Paper_evidenceWBPaper00036303
Curator_confirmedWBPerson2987
Remarknumr-1(ok2239) nematodes were egg-laying defective (Egl phenotype). This was demonstrated by an increase in the number of nematodes with a "bagging" phenotype, where nematodes fail to lay their eggs and embryos hatch inside the parent (Figure 6).Paper_evidenceWBPaper00036303
Curator_confirmedWBPerson2987
WBPhenotype:0000695Paper_evidenceWBPaper00036303
Curator_confirmedWBPerson2987
RemarkMicroscopic examination of the vulva in L4 numr-1(ok2239) nematodes showed that there were structural abnormalities, which may have contributed to the Egl phenotype (Figure 6). numr-1(ok2239) nematodes often lacked organized epithelial-ring structures that line the vulva lumen at the L4 stage.Paper_evidenceWBPaper00036303
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0006748PATO:0000460Paper_evidenceWBPaper00036303
Curator_confirmedWBPerson2987
Life_stageWBls:0000038PATO:0000460Paper_evidenceWBPaper00036303
Curator_confirmedWBPerson2987
WBPhenotype:0001655Paper_evidenceWBPaper00036303
Curator_confirmedWBPerson2987
RemarkRNAi-treated numr-1(ok2239) C. elegans that were exposed to 100 μM cadmium had a significantly reduced lifespan, compared with numr-1/-2 RNAi-treated wild-type nematodes (P = 0.0001; Figure 8).Paper_evidenceWBPaper00036303
Curator_confirmedWBPerson2987
WBPhenotype:0002241Paper_evidenceWBPaper00036303
Curator_confirmedWBPerson2987
Remarknumr-1(ok2239) nematodes did not feed as much (i.e. Eat phenotype) as the wild-type nematodes (Figure 6).Paper_evidenceWBPaper00036303
Curator_confirmedWBPerson2987
Phenotype_assayTreatmentBriefly, 25 adult C. elegans were dispensed into each well of a 96-well plate, containing K-medium and OP50 E. coli, using a COPAS Biosort (Union Biometrica Inc., Somerville, MA, USA). After 4 hours, Fluoresbrite polychromatic red microspheres (Polysciences, Inc., Warrington, PA) were added into each well and mixed for 5 minutes. Nematodes were allowed to ingest the microspheres for 10 additional minutes and then anesthetized by adding sodium azide (10 millimolar final concentration) to prevent additional bead ingestion. The size and level of fluorescence of individual C. elegans were measured.Paper_evidenceWBPaper00036303
Curator_confirmedWBPerson2987
Phenotype_not_observedWBPhenotype:0001653Paper_evidenceWBPaper00036303
Curator_confirmedWBPerson2987
RemarkTreatment of numr-1(ok2239) with cadmium did not significantly affect longevity, relative to wild-type nematodes. This may have been due to the presence of the intact numr-2 and continued NUMR-2 expression.Paper_evidenceWBPaper00036303
Curator_confirmedWBPerson2987
ReferenceWBPaper00036303
RemarkSequenced by the C. elegans Gene Knockout ConsortiumPaper_evidenceWBPaper00041807
MethodKO_consortium_allele