WormBase Tree Display for Variation: WBVar00093704

WBVar00093704 Evidence: Paper_evidence: WBPaper00036066
  Name: Public_name: ok2581
    Other_name: C09E8.3.1:c.1000-15_1504delinsC
    HGVSg: CHROMOSOME_II:g.549553_551323delinsC
  Sequence_details: SMap: S_parent: Sequence: C09E8
    Flanking_sequences: ccacaaatctcccagtacaaagtacaaatt tccgagttgctcgccgagccgacatcgtct
    Mapping_target: C09E8
    Type_of_mutation: Insertion: C
      Deletion
    PCR_product: ok2581_external
      ok2581_internal
    SeqStatus: Sequenced
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00032646
    Laboratory: RB
    Person: WBPerson46
    KO_consortium_allele
    Status: Live
  Affects: Gene: WBGene00015646
    Transcript: C09E8.3.1 VEP_consequence: splice_acceptor_variant,splice_donor_variant,coding_sequence_variant,intron_variant
        VEP_impact: HIGH
        HGVSc: C09E8.3.1:c.1000-15_1504delinsC
        cDNA_position: ?-1630
        CDS_position: ?-1504
        Protein_position: ?-502
        Intron_number: 5-8/9
        Exon_number: 6-9/10
  Isolation: Mutagen: EMS
  Description: Phenotype: WBPhenotype:0000137 Paper_evidence: WBPaper00036066
        Curator_confirmed: WBPerson2021
        Remark: Transcripts of mlt-10 were less abundant in ok2581 mutants than wild-type animals Paper_evidence: WBPaper00036066
            Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00036066
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000436 Paper_evidence: WBPaper00036066
        Curator_confirmed: WBPerson2021
        Remark: COL-19::GFP macromolecules were disorganized in many mlt-10(ok2581) animals Paper_evidence: WBPaper00036066
            Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00036066
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000638 Paper_evidence: WBPaper00036066
        Curator_confirmed: WBPerson2021
        Remark: Buccal caps were observed on the majority of mlt-10(ok2581) mutants compared to 20% of wild-type larvae. When cultured on low cholesterol media, 92% of mlt-10(ok2581) mutants failed to shed the fourth larval cuticle, compared to 29% of wild-type animals Paper_evidence: WBPaper00036066
            Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00036066
            Curator_confirmed: WBPerson2021
      WBPhenotype:0000899 Paper_evidence: WBPaper00036066
        Curator_confirmed: WBPerson2021
        Remark: Seam cells were misshapen and overlapped their sisters in mlt-10 mutants Paper_evidence: WBPaper00036066
            Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00036066
            Curator_confirmed: WBPerson2021
        EQ_annotations: Anatomy_term: WBbt:0005753 PATO:0000460 Paper_evidence: WBPaper00036066
                Curator_confirmed: WBPerson2021
      WBPhenotype:0001095 Paper_evidence: WBPaper00036066
        Curator_confirmed: WBPerson2021
        Remark: The survival rate of various mlt-10 mutants was significantly lower than that of wild-type animals on media containing 300 mM NaCl Paper_evidence: WBPaper00036066
            Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00036066
            Curator_confirmed: WBPerson2021
      WBPhenotype:0001211 Paper_evidence: WBPaper00036066
        Curator_confirmed: WBPerson2021
        Remark: Mutants were all more permeable to Hoechst than wild-type larvae (porous cuticles) Paper_evidence: WBPaper00036066
            Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00036066
            Curator_confirmed: WBPerson2021
      WBPhenotype:0001412 Paper_evidence: WBPaper00036066
        Curator_confirmed: WBPerson2021
        Remark: Segments of the alae were broken, branched, or comprised of four ridges Paper_evidence: WBPaper00036066
            Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00036066
            Curator_confirmed: WBPerson2021
      WBPhenotype:0001834 Paper_evidence: WBPaper00036066
        Curator_confirmed: WBPerson2021
        Remark: Transcripts of mlt-10 were shorter in ok2581 mutants than wild-type animals Paper_evidence: WBPaper00036066
            Curator_confirmed: WBPerson2021
        Variation_effect: Probable_null_via_phenotype: Paper_evidence: WBPaper00036066
            Curator_confirmed: WBPerson2021
  Reference: WBPaper00036066
  Remark: Sequenced by the C. elegans Gene Knockout Consortium Paper_evidence: WBPaper00041807
  Method: KO_consortium_allele