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WormBase Tree Display for Variation: WBVar00095033

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Name Class

WBVar00095033EvidencePaper_evidenceWBPaper00006343
NamePublic_nameoy21
Other_nameCE25046:p.Gln127Ter
K07A9.2.1:c.379C>T
HGVSgCHROMOSOME_IV:g.1832069C>T
Sequence_detailsSMapS_parentSequenceK07A9
Flanking_sequencesacggaacaggacgcgtcgaatcttattagaaggtgagattttcagttgaagagatcaaaa
Mapping_targetK07A9
Type_of_mutationSubstitutionct
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00031132
WBStrain00047810
LaboratoryPY
StatusLive
AffectsGeneWBGene00000553
TranscriptK07A9.2.1VEP_consequencestop_gained,splice_region_variant
VEP_impactHIGH
HGVScK07A9.2.1:c.379C>T
HGVSpCE25046:p.Gln127Ter
cDNA_position426
CDS_position379
Protein_position127
Exon_number4/9
Codon_changeCag/Tag
Amino_acid_changeQ/*
Interactor (22)
GeneticsInterpolated_map_positionIV-15.5054
DescriptionPhenotypeWBPhenotype:0000146Paper_evidenceWBPaper00049050
Curator_confirmedWBPerson2987
Remark"We also subjected AFD neurons of cmk-1 mutants to single-trial calcium imaging and found that response temperatures were abnormally perturbed at both 20 degreees Ceslius and 23 degreees Ceslius cultivation (Figures 2E and 2F; Figure S2C). While wild-type AFD neurons of 23 degreees Ceslius-cultivated animals initiated calcium responses within a temperature range of about 19.0 degreees Ceslius to 22.0 degreees Ceslius, cmk-1 mutant AFD neurons started to respond within a wider temperature range of about 16.0 degreees Ceslius to 21.0 degreees Ceslius(Figures 2E and 2F)... Similar to in vivo conditions, isolated cmk-1 mutant AFD neurons also showed increased variability in response temperature, suggesting that the lack of CMK-1 only in AFD is responsible for the abnormal variability in response temperature of AFD (Figure 2G). Indeed, expression of a cmk-1 cDNA only in AFD completely rescued the response temperatures in cmk-1 mutants, indicating that CMK-1 cell-autonomously acts in AFD (Figures 2E, 2F, and S2C)."Paper_evidenceWBPaper00049050
Curator_confirmedWBPerson2987
Rescued_by_transgeneWBTransgene00025153
EQ_annotationsAnatomy_termWBbt:0005662PATO:0000460Paper_evidenceWBPaper00049050
Curator_confirmedWBPerson2987
Phenotype_assayGenotypenjIs24[gcy-8p::GCaMP3, gcy-8p::TagRFP] (I); Parent strain: IK1013Paper_evidenceWBPaper00049050
Curator_confirmedWBPerson2987
WBPhenotype:0000478Paper_evidenceWBPaper00049050
Curator_confirmedWBPerson2987
Remark"While most of the wild-type animals migrated around the cultivation temperature, cmk-1 mutants dispersed over a wider range of temperatures (Figures 4A-4C). Expression of a cmk-1 cDNA only in AFD rescued this cmk-1 mutant phenotype, while the expression of a kinase-negative form (K52M) of cmk-1 cDNA failed to rescue the behavioral abnormalities (Figures 4B and 4C)."Paper_evidenceWBPaper00049050
Curator_confirmedWBPerson2987
Rescued_by_transgeneWBTransgene00025153
WBPhenotype:0000637Paper_evidenceWBPaper00048507
Curator_confirmedWBPerson10260
RemarkFigure 1C, cmk-1 mutants form dauers in the presence of live food.Paper_evidenceWBPaper00048507
Curator_confirmedWBPerson10260
WBPhenotype:0001236Paper_evidenceWBPaper00049891
Curator_confirmedWBPerson10038
RemarkQuoted from paper: "These results indicate that the CMK-1 signaling pathway acts basally to repress glr-1 transcription. Expression of cmk-1 cDNA specifically in GLR-1-expressing neurons rescues the increase in Pglr-1 activity observed in cmk-1(oy21) loss-of-function mutants (Fig 3H)"Paper_evidenceWBPaper00049891
Curator_confirmedWBPerson10038
Rescued_by_transgeneWBTransgene00026932
Phenotype_assayStrainWBStrain00047810Paper_evidenceWBPaper00049891
Curator_confirmedWBPerson10038
Control_strainWBStrain00047313Paper_evidenceWBPaper00049891
Curator_confirmedWBPerson10038
GenotypepzIs29 [Pglr-1::NLS-GFP::LacZ::unc-54 3'UTR]Paper_evidenceWBPaper00049891
Curator_confirmedWBPerson10038
WBPhenotype:0001278Paper_evidenceWBPaper00049050
Curator_confirmedWBPerson2987
Remark"In the suppressor screen, we utilized pleiotropic defects of cmk-1 mutants: cmk-1 mutants showed abnormally low expression of nhr-38 promoter-fused GFP, an AFD neuron-specific GFP marker (Satterlee et al., 2004)." (Figure S3A, S3B)Paper_evidenceWBPaper00049050
Curator_confirmedWBPerson2987
"Furthermore, these Raf pathway mutations also suppressed the abnormally low expression of the AFD thermosensory machinery gene gcy-8 in cmk-1 mutant background (Supplemental Results; Figure S4)." As measured by RFP fluorescence, where RFP expression is driven by the gcy-8 promoter (see genotype)Paper_evidenceWBPaper00049050
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0005662PATO:0000460Paper_evidenceWBPaper00049050
Curator_confirmedWBPerson2987
Phenotype_assayGenotypenjIs2[nhr-38p::GFP, AIYp::GFP] (V); Parent strain: IK0778Paper_evidenceWBPaper00049050
Curator_confirmedWBPerson2987
njIs24[gcy-8p::GCaMP3, gcy-8p::TagRFP] (I); Parent strain: IK1013Paper_evidenceWBPaper00049050
Curator_confirmedWBPerson2987
WBPhenotype:0002199Paper_evidenceWBPaper00049050
Curator_confirmedWBPerson2987
Remark"We subjected AFD neurons of 23 degree Celsius-cultivated cmk-1 mutants to six trials of calcium imaging and compared the variability to that of wild-type animals. While trial-to-trial variability in wild-type and cmk-1 animals were comparable, animal-to-animal variability in cmk-1 mutants was significantly larger than that of wild-type animals, showing that the lack of CMK-1 increases the animal-to-animal variability of AFD (Figure 2D)."Paper_evidenceWBPaper00049050
Curator_confirmedWBPerson2987
"We found that, similar to the case of AFD, the AIY neuron of cmk-1 mutants showed abnormally increased variability in response temperatures: while response temperatures of 23 degrees Celsius-cultivated wild-type AIY neurons were within a temperature range of about 16.8 degrees Celsius to 20.0 degrees Celsius, those of cmk-1 mutant AIY neurons were distributed within a wider temperature range of about 15.3 degrees Celsius to 20.1 degrees Celsius (Figure 5C). Expression of a cmk-1 cDNA only in AFD rescued these abnormal response temperatures of AIY in cmk-1 mutants, indicating that the CMK-1-mediated variability of AFD influences the variability of the AIY response (Figure 5C)."Paper_evidenceWBPaper00049050
Curator_confirmedWBPerson2987
Rescued_by_transgeneWBTransgene00025153
EQ_annotationsAnatomy_termWBbt:0005662PATO:0000460Paper_evidenceWBPaper00049050
Curator_confirmedWBPerson2987
WBbt:0005413PATO:0000460Paper_evidenceWBPaper00049050
Curator_confirmedWBPerson2987
Phenotype_assayGenotypenjIs24[gcy-8p::GCaMP3, gcy-8p::TagRFP] (I); Parent strain: IK1013Paper_evidenceWBPaper00049050
Curator_confirmedWBPerson2987
njIs26[AIYp::GCaMP3, AIYp::TagRFP, ges-1p::nls-TagRFP] (X); Parent strain: IK1273Paper_evidenceWBPaper00049050
Curator_confirmedWBPerson2987
ReferenceWBPaper00006343
WBPaper00046106
WBPaper00048507
WBPaper00049050
WBPaper00049891
MethodSubstitution_allele