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WormBase Tree Display for Variation: WBVar00145345

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Name Class

WBVar00145345EvidencePaper_evidenceWBPaper00031666
NamePublic_namefr4
Other_nameK09A9.1.1:c.920T>A
CE42581:p.Ile307Asn
HGVSgCHROMOSOME_X:g.15609073T>A
Sequence_detailsSMapS_parentSequenceK09A9
Flanking_sequencesatgaaataactgaaaacgacgtgatgagtactaccaaaaggttgtggagattgttcgatt
Mapping_targetK09A9
Type_of_mutationSubstitutiontaPaper_evidenceWBPaper00031666
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00021978
WBStrain00021982
LaboratoryIG
StatusLive
AffectsGeneWBGene00010700
TranscriptK09A9.1.1 (12)
InteractorWBInteraction000503573
WBInteraction000536530
WBInteraction000536534
GeneticsInterpolated_map_positionX22.9307
DescriptionPhenotypeWBPhenotype:0000039Paper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
RemarkLife span is decreased compared to wild type animals.Paper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAssayed on E. coli.Paper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
WBPhenotype:0000134Paper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
RemarkAnimals exhibit reduced expression of pnlp-29::GFP. Expression is rescued when nipi-3 is expressed in the epidermis and not the intestine.Paper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were infected with D. coniospora at the L4 stage and maintained on OP50 at 20C.Paper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
GenotypefrIs7Paper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
WBPhenotype:0000142Paper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
RemarkUpregulation of pnlp-29::GFP after infection with D. coniospora was abolished but animals still responded strongly to injury. In addition, nlp-29 and nlp-31 mRNA species were not induced upon infection but was similiarly induce compared to that in wild type animals upon wounding, as confirmed by qRT-PCR.Paper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were infected with D. coniospora at the L4 stage and maintained on OP50 at 20C. Animals were wounded by microinjection needle pricking of the cuticle or by femtosecond laser busts.Paper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
GenotypefrIs7Paper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
WBPhenotype:0000583Paper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
RemarkAnimals are 20-25% shorter than wild type worms as demonstrated by the mean time of flight (TOF) quantified with the Biosort Worm Sorter.Paper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Paper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
Phenotype_assayTemperature25Paper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
WBPhenotype:0001013Paper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
RemarkAnimals exhibit a significant reduction in survival when infected with D. coniospora.Paper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
RecessivePaper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were infected with D. coniospora at the L4 stage and maintained on OP50 at 20C.Paper_evidenceWBPaper00031666
Curator_confirmedWBPerson712
WBPhenotype:0001278Paper_evidenceWBPaper00041022
Curator_confirmedWBPerson2987
Remarknipi-3(fr4) reduced, but did not block, the induction of Pnlp-29::GFP expression induced by Pcol-19::GPA-12* (* activated form of GPA-12)Paper_evidenceWBPaper00041022
Curator_confirmedWBPerson2987
Phenotype_assayGenotypePnlp-29::GFP; Pcol-19::GPA-12* (* activated form of GPA-12)Paper_evidenceWBPaper00041022
Curator_confirmedWBPerson2987
WBPhenotype:0001800Paper_evidenceWBPaper00033094
Curator_confirmedWBPerson2021
Remarknipi-3(fr4) is required for the response of C. elegans to infectionPaper_evidenceWBPaper00033094
Curator_confirmedWBPerson2021
Phenotype_assayGenotypefrIs7 [pnlp-29::GFP]Paper_evidenceWBPaper00033094
Curator_confirmedWBPerson2021
Phenotype_not_observedWBPhenotype:0001838Paper_evidenceWBPaper00033094
Curator_confirmedWBPerson2021
RemarkUpon treatment with PMA, a robust upregulation of the nlp-29 reporter was seen in nipi-3(fr4) worms, similar to wild-typePaper_evidenceWBPaper00033094
Curator_confirmedWBPerson2021
Phenotype_assayGenotypefrIs7 [pnlp-29::GFP]Paper_evidenceWBPaper00033094
Curator_confirmedWBPerson2021
WBPhenotype:0001839Paper_evidenceWBPaper00033094
Curator_confirmedWBPerson2021
RemarkMutants were not resistant to PMAPaper_evidenceWBPaper00033094
Curator_confirmedWBPerson2021
Phenotype_assayGenotypefrIs7 [pnlp-29::GFP]Paper_evidenceWBPaper00033094
Curator_confirmedWBPerson2021
WBPhenotype:0001840Paper_evidenceWBPaper00033094
Curator_confirmedWBPerson2021
RemarkNormal increase in pnlp-29::GFP expression after woundingPaper_evidenceWBPaper00033094
Curator_confirmedWBPerson2021
Phenotype_assayGenotypefrIs7 [pnlp-29::GFP]Paper_evidenceWBPaper00033094
Curator_confirmedWBPerson2021
ReferenceWBPaper00041022
WBPaper00033094
WBPaper00031666
MethodSubstitution_allele