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WormBase Tree Display for Variation: WBVar00145576

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Name Class

WBVar00145576EvidencePaper_evidenceWBPaper00032399
NamePublic_namegk169
Other_nameF09E5.15c.1:c.18+195_216del
F09E5.15b.1:c.13-207_210del
HGVSgCHROMOSOME_II:g.5378635_5379039del
Sequence_detailsSMapS_parentSequenceF09E5
Flanking_sequencesctcatttcgtcctccgattttttttctttcaacaccgttgtgctcgccgcttccaccgac
Mapping_targetF09E5
Type_of_mutationDeletion
PCR_productgk169_external
gk169_internal
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00035650
LaboratoryVC
PersonWBPerson427
KO_consortium_allele
StatusLive
AffectsGeneWBGene00006434
TranscriptF09E5.15b.1VEP_consequencesplice_acceptor_variant,coding_sequence_variant,intron_variant
VEP_impactHIGH
HGVScF09E5.15b.1:c.13-207_210del
cDNA_position?-210
CDS_position?-210
Protein_position?-70
Intron_number1/2
Exon_number2/3
F09E5.15a.1VEP_consequencecoding_sequence_variant,5_prime_UTR_variant
VEP_impactMODIFIER
cDNA_position?-198
CDS_position?-198
Protein_position?-66
Exon_number1/3
F09E5.15c.1VEP_consequencesplice_acceptor_variant,coding_sequence_variant,intron_variant
VEP_impactHIGH
HGVScF09E5.15c.1:c.18+195_216del
cDNA_position?-216
CDS_position?-216
Protein_position?-72
Intron_number1/2
Exon_number2/3
InteractorWBInteraction000503847
WBInteraction000520387
IsolationMutagenUV/TMP
DescriptionPhenotypeWBPhenotype:0000119Paper_evidenceWBPaper00046711
Curator_confirmedWBPerson3979
Remarkprdx-2 (gk169) contains increased nuclear levels of SKN-1 and DAF-16 transcription factors.Paper_evidenceWBPaper00046711
Curator_confirmedWBPerson3979
WBPhenotype:0000135Paper_evidenceWBPaper00032399
Curator_confirmedWBPerson712
RemarkRelative mRNA levels of gcs-1 and gst-1, both phase II detoxification genes, were elevated compared with levels in N2 animals. Levels of total glutathione was also increased compared to wild type.Paper_evidenceWBPaper00032399
Curator_confirmedWBPerson712
WBPhenotype:0000154Paper_evidenceWBPaper00032399
Curator_confirmedWBPerson712
RemarkAnimals exhibited lowered fecundity compared to N2 animals, as demonstrated by smaller brood sizes.Paper_evidenceWBPaper00032399
Curator_confirmedWBPerson712
WBPhenotype:0000591Paper_evidenceWBPaper00032399
Curator_confirmedWBPerson712
RemarkAnimals exhibited increased resistance to oxidative-stress causing arsenite (As), and cadmium toxicity.Paper_evidenceWBPaper00032399
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00003022Paper_evidenceWBPaper00032399
Curator_confirmedWBPerson712
WBMol:00004596Paper_evidenceWBPaper00032399
Curator_confirmedWBPerson712
Phenotype_assayTreatmentAnimals were treated with 10mM Arsenite.Paper_evidenceWBPaper00032399
Curator_confirmedWBPerson712
WBPhenotype:0001171Paper_evidenceWBPaper00032399
WBPaper00049105
Curator_confirmedWBPerson712
WBPerson2987
RemarkAnimals exhibited reduced mean and median lifespans under various conditions (i.e. temperature and food source) compared to N2 animals.Paper_evidenceWBPaper00032399
Curator_confirmedWBPerson712
"To test a function of mitochondrial stress signaling in tumor survival during starvation, we used mutants in this signaling pathway, prdx-2(gk169) and aak-2(ok425). After induction of tumor formation by gld-1 RNAi, we performed life span analysis either under feeding (aak-2 and prdx-2; Figure 9B and S6A) or starvation conditions (aak-2 only; Figs 9C and S6B)... Notably, life span under feeding in animals with tumors depends on both aak-2 and prdx-2 function (Fig 9B), whereas starvation-induced life span extension is not affected in the aak-2 mutant (Fig 9C), consistent with recent findings."Paper_evidenceWBPaper00049105
Curator_confirmedWBPerson2987
Phenotype_assayTreatmentAnimals were grown on live OP50 or heat-killed OP50.Paper_evidenceWBPaper00032399
Curator_confirmedWBPerson712
Feeding conditionsPaper_evidenceWBPaper00049105
Curator_confirmedWBPerson2987
Temperature15,20Paper_evidenceWBPaper00032399
Curator_confirmedWBPerson712
Genotypegld-1(RNAi)Paper_evidenceWBPaper00049105
Curator_confirmedWBPerson2987
WBPhenotype:0001621Paper_evidenceWBPaper00032399
Curator_confirmedWBPerson712
RemarkAnimals showed reduced survival after to exposure to 1.0mM hydrogen peroxide compared to wild-type animals; this concentration was sublethal to wild-type animals.Paper_evidenceWBPaper00032399
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00001695Paper_evidenceWBPaper00032399
Curator_confirmedWBPerson712
WBPhenotype:0001739Paper_evidenceWBPaper00032399
Curator_confirmedWBPerson712
RemarkAnimals exhibited a more rapid accumulation of the age-associated fluorescent pigment, lipofuscin, in the gut, and a more rapid decline in motility than wild-type animals.Paper_evidenceWBPaper00032399
Curator_confirmedWBPerson712
WBPhenotype:0002382Paper_evidenceWBPaper00048427
Curator_confirmedWBPerson11689
Phenotype_assayGenotypedvIs19 [Pgst-4::GFP::NLS]Paper_evidenceWBPaper00048427
Curator_confirmedWBPerson11689
Phenotype_not_observedWBPhenotype:0000039Paper_evidenceWBPaper00050972
Curator_confirmedWBPerson2563
Remarktested in monoxenic and axenic mediumPaper_evidenceWBPaper00050972
Curator_confirmedWBPerson2563
WBPhenotype:0000146Paper_evidenceWBPaper00032399
Curator_confirmedWBPerson712
RemarkAnimals exhibited a survival rate at 37C similar to wild-type animals under the same conditions.Paper_evidenceWBPaper00032399
Curator_confirmedWBPerson712
ReferenceWBPaper00032399
WBPaper00046711
WBPaper00048427
WBPaper00049105
WBPaper00050972
WBPaper00065026
RemarkSequenced by the C. elegans Gene Knockout ConsortiumPaper_evidenceWBPaper00041807
MethodKO_consortium_allele