WormBase Tree Display for Variation: WBVar00250823

WBVar00250823 Name: Public_name: tm1860
    Other_name: R09A1.1a.1:c.1126+448_1391del
    HGVSg: CHROMOSOME_V:g.1011227_1012384del
  Sequence_details: SMap: S_parent: Sequence: R09A1
    Flanking_sequences: tattatatgaacacaaaattctgagaatgc acggaatttcaagtttgtcgggctcggagc
    Mapping_target: R09A1
    Source_location: 7 CHROMOSOME_V 1011226 1012385 Inferred_automatically: National_Bioresource_Project
    Type_of_mutation: Deletion
    PCR_product: tm1860_external
      tm1860_internal
    SeqStatus: Sequenced
  Variation_type: Allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00040450
    Laboratory: FX
    Author: Mitani S
    DB_info: Database: National_Bioresource_Project seq 1860
    NBP_allele
    Status: Live
  Affects: Gene: WBGene00019971
    Transcript: R09A1.1b.1 VEP_consequence: coding_sequence_variant,5_prime_UTR_variant
        VEP_impact: MODIFIER
        cDNA_position: ?-170
        CDS_position: ?-170
        Protein_position: ?-57
        Exon_number: 1/5
      R09A1.1a.1 VEP_consequence: splice_acceptor_variant,coding_sequence_variant,intron_variant
        VEP_impact: HIGH
        HGVSc: R09A1.1a.1:c.1126+448_1391del
        cDNA_position: ?-1401
        CDS_position: ?-1391
        Protein_position: ?-464
        Intron_number: 3/8
        Exon_number: 4/9
    Interactor: WBInteraction000521281
      WBInteraction000521418
  Isolation: Mutagen: TMP/UV
  Genetics: Map: V
  Description: Phenotype: WBPhenotype:0000136 Paper_evidence: WBPaper00035324
        Curator_confirmed: WBPerson2021
        Remark: Only targets of class II oocyte/embryo 26G RNAs were upregulated in the ergo-1(tm1860) mutant Paper_evidence: WBPaper00035324
            Curator_confirmed: WBPerson2021
      WBPhenotype:0001258 Paper_evidence: WBPaper00044603
        Curator_confirmed: WBPerson712
        Remark: transgene expression was silenced; wild-type animals express the transgene Paper_evidence: WBPaper00044603
            Curator_confirmed: WBPerson712
        Phenotype_assay: Genotype: mgIs30 Paper_evidence: WBPaper00044603
              Curator_confirmed: WBPerson712
      WBPhenotype:0001776 Paper_evidence: WBPaper00035324
        Curator_confirmed: WBPerson2021
        Remark: ergo-1 is required for the expression of class II oocyte/embryo 26G RNAs Paper_evidence: WBPaper00035324
            Curator_confirmed: WBPerson2021
    Phenotype_not_observed: WBPhenotype:0000039 Paper_evidence: WBPaper00049012
        Curator_confirmed: WBPerson11446
        Remark: these mutant worms all have normal life spans Paper_evidence: WBPaper00049012
            Curator_confirmed: WBPerson11446
      WBPhenotype:0000062 Person_evidence: WBPerson7743
        Curator_confirmed: WBPerson48
      WBPhenotype:0001759 Paper_evidence: WBPaper00031962
        Curator_confirmed: WBPerson712
        Remark: Levels of 21U-RNA, from Nothern blot or from qRT-PRC, were comparable to that of wild-type. Paper_evidence: WBPaper00031962
            Curator_confirmed: WBPerson712
        EQ_annotations: GO_term: GO:0034585 PATO:0000460 Paper_evidence: WBPaper00031962
                Curator_confirmed: WBPerson712
  Reference: WBPaper00031962
    WBPaper00035324
    WBPaper00044603
    WBPaper00049012
  Remark: 7750/7751-8908/8909 (1158 bp deletion)
    This knockout was generated by the National Bioresource Project, Tokyo, Japan, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. Paper_evidence: WBPaper00041807
  Method: NBP_knockout_allele