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WormBase Tree Display for Variation: WBVar00251364

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Name Class

WBVar00251364NamePublic_nametm2482
Other_nameR74.3.1:c.233_384del
R74.3.2:c.233_384del
CE40514:p.Ala78GlyfsTer12
HGVSgCHROMOSOME_III:g.4194215_4194416del
Sequence_detailsSMapS_parentSequenceR74
Flanking_sequencesaacttaaaaatcgagtcgcagcccaaaatggaagagaacaacgaaaacttgatgaacagc
Mapping_targetR74
Source_location7CHROMOSOME_III41942144194417Inferred_automaticallyNational_Bioresource_Project
Type_of_mutationDeletion
PCR_producttm2482_external
tm2482_internal
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
LaboratoryFX
AuthorMitani S
DB_infoDatabaseNational_Bioresource_Projectseq2482
NBP_allele
StatusLive
AffectsGeneWBGene00006959
TranscriptR74.3.1 (11)
R74.3.2 (11)
InteractorWBInteraction000536438
WBInteraction000536440
WBInteraction000536441
WBInteraction000536442
IsolationMutagenTMP/UV
GeneticsMapIII
DescriptionPhenotypeWBPhenotype:0000436Paper_evidenceWBPaper00046107
Curator_confirmedWBPerson10038
RemarkFrom the text: "In xbp-1(tm2482) null mutants (Richardson et al. 2011), Venus::UNC-6 accumulated in the neuronal cell body, as in the ire-1 mutants (Fig. 1H, I). Expression of the activated spliced form of xbp-1 restored the normal distribution of Venus::UNC-6 in both the ire-1 and xbp-1 mutants (Shim et al. 2004; Fig. 1F, G, J, K). These results suggest that xbp-1 splicing is essential for IRE-1-mediated UNC-6 localization. Thus, the IRE-1/XBP-1 pathway is critical for proper axonal distribution of Venus::UNC-6."Paper_evidenceWBPaper00046107
Curator_confirmedWBPerson10038
From the text: "However, when the glr-1 promoter was used to drive neuronal unc-6 gene expression in unc-6; ire-1 and unc-6; xbp-1 double mutants, UNC-6 was observed only in cell bodies (Fig. 2F, H)."Paper_evidenceWBPaper00046107
Curator_confirmedWBPerson10038
Phenotype_assayGenotypeghIs9[unc-6p::Venus::unc-6; str-3p::dsRed2] IVPaper_evidenceWBPaper00046107
Curator_confirmedWBPerson10038
unc-6(ev400); [ghEx15(glr-1p::unc-6::Venus; tph-1p::GFP)]Paper_evidenceWBPaper00046107
Curator_confirmedWBPerson10038
WBPhenotype:0001090Paper_evidenceWBPaper00040453
Curator_confirmedWBPerson2987
Remark"... we observed that the xbp-1 mutant exhibited larval lethality when grown at 27C, an elevated temperature at which WT N2 C. elegans exhibits a reduced brood size and increased dauer formation (Figure 5C)."Paper_evidenceWBPaper00040453
Curator_confirmedWBPerson2987
WBPhenotype:0001350Paper_evidenceWBPaper00040453
Curator_confirmedWBPerson2987
Remarkxbp-1(tm2482) resulted in increased levels of phosphorylated eIF2α (Figure 2B, S1B)Paper_evidenceWBPaper00040453
Curator_confirmedWBPerson2987
Phenotype_not_observedWBPhenotype:0000062Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
RemarkClassified as homozygous viable by the National Bioresource Project of Japan.Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
Laboratory_evidenceFX
WBPhenotype:0001012Paper_evidenceWBPaper00040453
Curator_confirmedWBPerson2987
Remark"Likewise, the larval development of the xbp-1 mutant, which is severely compromised on P. aeruginosa at 25C [21], was equivalent to that of WT at 16C (Figure 5A)."Paper_evidenceWBPaper00040453
Curator_confirmedWBPerson2987
Phenotype_assayTemperature16Paper_evidenceWBPaper00040453
Curator_confirmedWBPerson2987
ReferenceWBPaper00040453
WBPaper00046107
Remark10355/10356-10557/10558 (202 bp deletion)
This knockout was generated by the National Bioresource Project, Tokyo, Japan, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
MethodNBP_knockout_allele