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WormBase Tree Display for Variation: WBVar00251560

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Name Class

WBVar00251560NamePublic_nametm2715
Other_nameC02F5.4.1:c.157_610del
CE38771:p.His53SerfsTer32
HGVSgCHROMOSOME_III:g.8244644_8245186del
Sequence_detailsSMapS_parentSequenceC02F5
Flanking_sequencesgttgtatttcagacagtaaagaaagaaacaagttgcagtcagttcgaagttcgcaagaag
Mapping_targetC02F5
Source_location7CHROMOSOME_III82446438245187Inferred_automaticallyNational_Bioresource_Project
Type_of_mutationDeletion
PCR_producttm2715_external
tm2715_internal
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
LaboratoryFX
AuthorMitani S
DB_infoDatabaseNational_Bioresource_Projectseq2715
NBP_allele
StatusLive
AffectsGeneWBGene00015347
TranscriptC02F5.4.1 (11)
IsolationMutagenTMP/UV
GeneticsMapIII
DescriptionPhenotypeWBPhenotype:0000028Paper_evidenceWBPaper00032289
Curator_confirmedWBPerson712
RemarkPre-mRNA was not cleaved normally, as assayed by qRT-PCR of lin-15A; in addition, transcription termination failed to occur, as assayed by qRT-PCR of lin-15B.Paper_evidenceWBPaper00032289
Curator_confirmedWBPerson712
WBPhenotype:0000050Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
RemarkOriginally classified as 'homozygous viable' by the National Bioresource Project of Japan. Comments to the National Bioresource Project of Japan: Dr. T. Blumenthal: at 15 C: ~11% dead egg phenotype, at 20 C: brood size normal and no embryo lethality, at 25 C; ~32% dead egg phenotype; Dr. T. Blumenthal: PNAS 105, 16665 (2008).Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
Laboratory_evidenceMH
BL
PenetranceIncompletePerson_evidenceWBPerson7743
Curator_confirmedWBPerson712
Temperature_sensitiveHeat_sensitive25Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
Cold_sensitive15Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
Phenotype_assayTemperature15, 20, 25Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
WBPhenotype:0000136Paper_evidenceWBPaper00032289
Curator_confirmedWBPerson712
Remarklin-15A transcripts were increased, as assayed by qRT-PCR.Paper_evidenceWBPaper00032289
Curator_confirmedWBPerson712
WBPhenotype:0001175Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
RemarkComments to the National Bioresource Project of Japan: Dr. T. Blumenthal: at 25 C; ~4% male.Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
Laboratory_evidenceBL
Temperature_sensitiveHeat_sensitive25Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0000030Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
RemarkComments to the National Bioresource Project of Japan: Dr. M. Han: wild-type growth.Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
Laboratory_evidenceMH
WBPhenotype:0000688Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
RemarkComments to the National Bioresource Project of Japan: Dr. M. Han: fertile.Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
Laboratory_evidenceMH
WBPhenotype:0000699Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
RemarkComments to the National Bioresource Project of Japan: Dr. M. Han: normal vulval development.Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
Laboratory_evidenceMH
ReferenceWBPaper00032289
Remark18178/18179-18721/18722 (543 bp deletion)
This knockout was generated by the National Bioresource Project, Tokyo, Japan, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
MethodNBP_knockout_allele