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WormBase Tree Display for Variation: WBVar00251625

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Name Class

WBVar00251625NamePublic_nametm2793
Other_nameB0432.4.1:c.59+19_204del
HGVSgCHROMOSOME_II:g.286710_286912del
Sequence_detailsSMapS_parentSequenceB0432
Flanking_sequencesaggaaccgctgggtgggttttcggaatattaatggactttctgccggacttttgcgtcaa
Mapping_targetB0432
Source_location7CHROMOSOME_II286709286913Inferred_automaticallyNational_Bioresource_Project
Type_of_mutationDeletion
PCR_producttm2793_external
tm2793_internal
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
LaboratoryFX
AuthorMitani S
DB_infoDatabaseNational_Bioresource_Projectseq2793
NBP_allele
StatusLive
AffectsGeneWBGene00015186
TranscriptB0432.4.1VEP_consequencesplice_acceptor_variant,coding_sequence_variant,intron_variant
VEP_impactHIGH
HGVScB0432.4.1:c.59+19_204del
cDNA_position?-212
CDS_position?-204
Protein_position?-68
Intron_number2/6
Exon_number3/7
InteractorWBInteraction000501093
IsolationMutagenTMP/UV
GeneticsMapII
DescriptionPhenotypeWBPhenotype:0000241Paper_evidenceWBPaper00038273
Curator_confirmedWBPerson712
RemarkA two-fold increase was observed in the number of apoptotic corpses per gonad arm.Paper_evidenceWBPaper00038273
Curator_confirmedWBPerson712
WBPhenotype:0000463Paper_evidenceWBPaper00038273
Curator_confirmedWBPerson712
RemarkA 6% reduction was observed in glucose concentration in misc-1 mutants compared to control animals.Paper_evidenceWBPaper00038273
Curator_confirmedWBPerson712
WBPhenotype:0000823Paper_evidenceWBPaper00038273
Curator_confirmedWBPerson712
RemarkAbsence of MISC-1 greatly increases the number of pH3-positive (histone 3 phosphorylation) mitotic germline stem cells and causes an extended mitotic zone (data not shown).Paper_evidenceWBPaper00038273
Curator_confirmedWBPerson712
WBPhenotype:0001401Paper_evidenceWBPaper00038273
Curator_confirmedWBPerson712
RemarkWhen analyzed by transmission electron microscopy, morphological defects in the mitochondria are observed. The mitochondria of misc-1 worms are smaller and more spherical. High magnification images indicate that compared to N2 controls, the mitochondrial cristae of the misc-1 mutant are more disorganized, possess increased blebbing characteristics and adopt more irregular orientations. Furthermore, the cristae of misc-1 animals are fewer in number.Paper_evidenceWBPaper00038273
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0000033Paper_evidenceWBPaper00038273
Curator_confirmedWBPerson712
RemarkHomozygous animals have the same development time as wild type (measured as time from egg laying to day one of adulthood).Paper_evidenceWBPaper00038273
Curator_confirmedWBPerson712
WBPhenotype:0000039Paper_evidenceWBPaper00038273
Curator_confirmedWBPerson712
RemarkHomozygous animals have normal life span (data not shown).Paper_evidenceWBPaper00038273
Curator_confirmedWBPerson712
WBPhenotype:0000062Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
RemarkClassified as homozygous viable by the National Bioresource Project of Japan.Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
Laboratory_evidenceFX
WBPhenotype:0000520Paper_evidenceWBPaper00038273
Curator_confirmedWBPerson712
RemarkHomozygous animals are morphologically normal.Paper_evidenceWBPaper00038273
Curator_confirmedWBPerson712
WBPhenotype:0001901Paper_evidenceWBPaper00038273
Curator_confirmedWBPerson712
RemarkHomozygous animals have normal fat staining.Paper_evidenceWBPaper00038273
Curator_confirmedWBPerson712
ReferenceWBPaper00038273
Remark29349/29350-29552/29553 (203 bp deletion)
This knockout was generated by the National Bioresource Project, Tokyo, Japan, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
MethodNBP_knockout_allele