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WormBase Tree Display for Variation: WBVar00251726

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Name Class

WBVar00251726NamePublic_nametm2913
Other_nameY113G7A.5.1:c.295_515del
CE32005:p.Tyr99ThrfsTer44
HGVSgCHROMOSOME_V:g.20029029_20029325del
Sequence_detailsSMapS_parentSequenceY113G7A
Flanking_sequencestccagaatcctgaccgagttgctcagtaaaaccgaatgtatgtatagtcaactcaaaagg
Mapping_targetY113G7A
Source_location7CHROMOSOME_V2002902820029326Inferred_automaticallyNational_Bioresource_Project
Type_of_mutationDeletion
PCR_producttm2913_external
tm2913_internal
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
LaboratoryFX
AuthorMitani S
DB_infoDatabaseNational_Bioresource_Projectseq2913
NBP_allele
StatusLive
AffectsGeneWBGene00013746
TranscriptY113G7A.5.1 (11)
IsolationMutagenTMP/UV
GeneticsMapV
DescriptionPhenotypeWBPhenotype:0000228Paper_evidenceWBPaper00033406
Curator_confirmedWBPerson2987
Remark"... we found that lgc-55 mutants had an increase in spontaneous reversals compared to the wild-type , although the increase was less pronounced than that of the tdc-1 mutants ( Figure 7B ) ."Paper_evidenceWBPaper00033406
Curator_confirmedWBPerson2987
WBPhenotype:0000315Paper_evidenceWBPaper00048522
WBPaper00033406
Curator_confirmedWBPerson2987
Remark"In response to touch, wild-type animals suppressed head movements by relaxing their head (Δhead = 5 0.001 μm, n = 39) and reversed on average 3.14 0.18 backward body bends (n = 100) (Fig 6, S4 Fig, S3 Movie). lgc-55 null mutant animals made shorter reversals than the wild type and fail to suppress the exploratory head movements during the reversal, with no significant change in head length (2.45 0.15 backward body bends, n = 100)."Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
"... we found that lgc-55 mutant animals failed to suppress head oscillations in response to anterior touch ( Figure 5 ; Movie S5 ) ."Paper_evidenceWBPaper00033406
Curator_confirmedWBPerson2987
" Like tdc-1 mutants , lgc-55 mutants initiated backward locomotion normally in response to anterior touch but displayed shorter runs of backward movement ( 2.4 +/- 0.1 body bends ) than the wild-type ( 3.3 +/- 0.1 body bends ) ( Figures 7A and S6 ) ."Paper_evidenceWBPaper00033406
Curator_confirmedWBPerson2987
WBPhenotype:0000679Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
Remark"Synaptic markers also properly localized in tyramine-deficient, tdc-1 mutants and lgc-55 null mutants (Fig 3B and S3 Fig). The postsynaptic densities were expanded in tyramine-deficient animals, whereas presynaptic densities were enlarged in the tyramine receptor mutants. The RIM-AVB synaptic markers were slightly more diffuse in tyramine signaling mutants and the LGC-55 cation-II transgenic animals."Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
EQ_annotationsAnatomy_termWBbt:0005835PATO:0000460Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
WBbt:0005841PATO:0000460Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
Phenotype_assayGenotypezfIs61 [Pcex-1::mCherry::RAB-3; LIN-15(+)]Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
WBPhenotype:0001005Paper_evidenceWBPaper00039982
Curator_confirmedWBPerson712
RemarkMutants reverse normally in response to touch; however,mutant animals back up slightly less far than the wild-type.Paper_evidenceWBPaper00039982
Curator_confirmedWBPerson712
WBPhenotype:0002134Paper_evidenceWBPaper00039982
Curator_confirmedWBPerson712
Remarklgc-55 mutants initiated reversals when wedged in a constricting ring trap of Drechslerella spp.: D. dactyloides, but escaped less frequently than wild-type animals.Paper_evidenceWBPaper00039982
Curator_confirmedWBPerson712
WBPhenotype:0002320Paper_evidenceWBPaper00033406
Curator_confirmedWBPerson2987
Remark"We found that lgc-55 mutants displayed more short reversals than wild-type worms ( Figure 7C ) . The increase in short reversals was even more dramatic in the tdc-1 mutants . An lgc-55 minigene rescued the defects of lgc-55 mutants in reversal behavior in both the touch-induced and spontaneous reversal assays ."Paper_evidenceWBPaper00033406
Curator_confirmedWBPerson2987
WBPhenotype:0002366Paper_evidenceWBPaper00039982
WBPaper00048522
Curator_confirmedWBPerson712
WBPerson2987
Remarklgc-55 mutant animals fail to suppress exploratory head movements during touch-induced reversals.Paper_evidenceWBPaper00039982
Curator_confirmedWBPerson712
"In response to touch, wild-type animals suppressed head movements by relaxing their head (Δhead = 5 0.001 μm, n = 39) and reversed on average 3.14 0.18 backward body bends (n = 100) (Fig 6, S4 Fig, S3 Movie). lgc-55 null mutant animals made shorter reversals than the wild type and fail to suppress the exploratory head movements during the reversal, with no significant change in head length (2.45 0.15 backward body bends, n = 100)."Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
WBPhenotype:0002367Paper_evidenceWBPaper00039982
Curator_confirmedWBPerson712
RemarkOnce mutants entered the holes in a nylon mesh, the animals took more than twice as long to extricate themselves as did wild-type animals.Paper_evidenceWBPaper00039982
Curator_confirmedWBPerson712
WBPhenotype:0002399Paper_evidenceWBPaper00048522
WBPaper00033406
Curator_confirmedWBPerson2987
Remark"Exogenous tyramine induced neck muscle relaxation and lengthening of the head in wild-type (LGC-55 anion) animals and lgc-55 null mutant animals that express a rescuing LGC-55 anion transgene (wild type: Δhead = 10 6 μm, n = 68; Plgc-55:LGC-55(zfEx2): Δhead = 11 4 μm, n = 75) (Fig 5A and 5B). Head movements persisted in lgc-55 mutants [19], with no significant change in head length (lgc-55(tm2913): Δhead = 3 13 μm, n = 65)."Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
"In wild-type animals, exogenous tyramine also induced long backward runs preceding immobilization through the LGC-55 mediated inhibition of the AVB premotor interneurons that drive forward locomotion (wild type: Δfwd-bwd = -8.46 2.98 body bends, n = 40) (Fig 5A and 5D, S1 Movie) [19]. Backward locomotion was further increased in transgenic animals that expressed the LGC-55 anion under control of its endogenous promoter (Plgc-55:LGC-55 (zfEx2): Δfwd-bwd = -28.5 3.3 backward body bends, n = 29). lgc-55 null mutants did not make long reversals when exposed to exogenous tyramine (lgc-55(tm2913): Δfwd-bwd = 2.58 0.9 body bends, n = 34)."Paper_evidenceWBPaper00048522
Curator_confirmedWBPerson2987
"Like lgc-55 ( zf11 ) and lgc-55 ( zf53 ) mutants , lgc-55 ( tm2913 ) mutants animals are resistant to tyramine-mediated head paralysis , suggesting that lgc-55 ( zf11 ) and lgc-55 ( zf53 ) also represent loss-of-function alleles. We were able to restore tyramine sensitivity by expressing a transgenic lgc-55 minigene in lgc-55 mutants ( Figure 1 C ) . These data indicate that lgc-55 is required for the paralytic effects of exogenous tyramine on head movements."Paper_evidenceWBPaper00033406
Curator_confirmedWBPerson2987
Affected_byMoleculeWBMol:00001396Paper_evidenceWBPaper00048522
WBPaper00033406
Curator_confirmedWBPerson2987
Phenotype_not_observedWBPhenotype:0000033Paper_evidenceWBPaper00039982
Curator_confirmedWBPerson712
Remarklgc-55 mutant animals are indistinguishable from the wild-type with respect to developmental timing.Paper_evidenceWBPaper00039982
Curator_confirmedWBPerson712
WBPhenotype:0000062Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
RemarkClassified as homozygous viable by the National Bioresource Project of Japan.Person_evidenceWBPerson7743
Curator_confirmedWBPerson712
Laboratory_evidenceFX
WBPhenotype:0000231Paper_evidenceWBPaper00039982
Curator_confirmedWBPerson712
Remarklgc-55 mutant animals are indistinguishable from the wild-type with respect to size.Paper_evidenceWBPaper00039982
Curator_confirmedWBPerson712
WBPhenotype:0000643Paper_evidenceWBPaper00039982
Curator_confirmedWBPerson712
Remarklgc-55 mutant animals are indistinguishable from the wild-type with respect to locomotion rate and locomotion pattern.Paper_evidenceWBPaper00039982
Curator_confirmedWBPerson712
WBPhenotype:0000662Paper_evidenceWBPaper00039982
Curator_confirmedWBPerson712
Remarklgc-55 mutant animals are indistinguishable from the wild-type with respect to exploratory behavior.Paper_evidenceWBPaper00039982
Curator_confirmedWBPerson712
WBPhenotype:0001101Paper_evidenceWBPaper00038382
Curator_confirmedWBPerson712
RemarkNo effects on clozapine-induced egg laying were observed.Paper_evidenceWBPaper00038382
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00003634Paper_evidenceWBPaper00038382
Curator_confirmedWBPerson712
WBPhenotype:0002026Paper_evidenceWBPaper00039982
Curator_confirmedWBPerson712
Remarklgc-55 mutant animals are indistinguishable from the wild-type with respect to touch sensitivity.Paper_evidenceWBPaper00039982
Curator_confirmedWBPerson712
ReferenceWBPaper00038382
WBPaper00039982
WBPaper00033406
WBPaper00048522
Remark2603/2604-2900/2901 (297 bp deletion)
This knockout was generated by the National Bioresource Project, Tokyo, Japan, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
MethodNBP_knockout_allele