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WormBase Tree Display for Variation: WBVar00296645

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Name Class

WBVar00296645NamePublic_nameok3692
Other_name (16)
HGVSgCHROMOSOME_V:g.13173799_13174455del
Sequence_detailsSMapS_parentSequenceT09E8
Flanking_sequencesaacttgaactgacacaaaaagaacagagctgaatttgagagaatgcgatcagagaaagga
Mapping_targetT09E8
Type_of_mutationDeletion
PCR_productok3692_external
ok3692_internal
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00037614
LaboratoryRB
PersonWBPerson46
KO_consortium_allele
StatusLive
AffectsGeneWBGene00011647
TranscriptT09E8.1d.1 (11)
T09E8.1c.1 (11)
T09E8.1a.1 (11)
T09E8.1g.1 (11)
T09E8.1e.1 (11)
T09E8.1h.1 (11)
T09E8.1f.1 (11)
T09E8.1b.1 (11)
IsolationMutagenEMS
DescriptionPhenotypeWBPhenotype:0000036Paper_evidenceWBPaper00048540
Curator_confirmedWBPerson12586
RemarkAt 72-hour after bleach synchronization and release, worms that are homozygous for both lt1 and ok3692 have a body length that is only ~50% of wild type or single mutant controls.Paper_evidenceWBPaper00048540
Curator_confirmedWBPerson12586
WBPhenotype:0000058Paper_evidenceWBPaper00048540
Curator_confirmedWBPerson12586
RemarkWorms that are homozygous for both lt1 and ok3692 rupture and die starting from L4 stage. ~15% of worms die at L4 stage after L1 release from bleach synchronization.Paper_evidenceWBPaper00048540
Curator_confirmedWBPerson12586
WBPhenotype:0000060Paper_evidenceWBPaper00048540
Curator_confirmedWBPerson12586
RemarkAbout 60% of worms that are homozygous for both lt1 and ok3692 rupture and die at 72-hour after L1 release from bleach synchronization. This is the 1st day adult.Paper_evidenceWBPaper00048540
Curator_confirmedWBPerson12586
WBPhenotype:0000685Paper_evidenceWBPaper00048540
Curator_confirmedWBPerson12586
RemarkWorms that are homozygous for both lt1 and ok3692 grow slower than wild type or single mutant controls. At the L4 stage, their body length are only about 2/3 of wild type or single mutant controls.Paper_evidenceWBPaper00048540
Curator_confirmedWBPerson12586
WBPhenotype:0000688Paper_evidenceWBPaper00048540
Curator_confirmedWBPerson12586
RemarkHomozygous ok3692 worms grew up to be normal size adults that are completely sterile. ok3692 allele is balanced by nT1[qIs51], but its locus is ~2 cM outside of the translocation junction. Thus, recombination could happen at a low frequency. It is thus important to maintain the allele by frequently singling out non-sterile carrying the green pharynx marker and verify correct segregation. Note that the original ok3692 allele from CGC is linked with a daf-c mutation. Please contact the Oegema lab to obtain the outcrossed allele.Paper_evidenceWBPaper00048540
Curator_confirmedWBPerson12586
ReferenceWBPaper00048540
RemarkSequenced by the C. elegans Gene Knockout ConsortiumPaper_evidenceWBPaper00041807
MethodKO_consortium_allele