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WormBase Tree Display for Variation: WBVar02125774

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Name Class

WBVar02125774EvidencePaper_evidenceWBPaper00041219
NamePublic_namevt21
Other_nameT23G5.5.1:c.1379G>A
CE25124:p.Gly460Asp
HGVSgCHROMOSOME_III:g.9245369G>A
Sequence_detailsSMapS_parentSequenceT23G5
Flanking_sequencesaaaactatcttcatcaaaaatttttgaaggtggaattctaatcatggaatggctcatcat
Mapping_targetT23G5
Type_of_mutationSubstitutiongaPaper_evidenceWBPaper00041219
SeqStatusSequenced
Variation_typeAllele
OriginSpeciesCaenorhabditis elegans
LaboratoryBY
StatusLive
AffectsGeneWBGene00000934
TranscriptT23G5.5.1 (12)
InteractorWBInteraction000521891
GeneticsInterpolated_map_positionIII0.362032
DescriptionPhenotypeWBPhenotype:0000273Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
RemarkMutants recovered in the screen demonstrated significantly reduced maximal thrashing frequencies in water.Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
WBPhenotype:0000536Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
RemarkOur assay of [3H]DA transport activity in COS-7 cells revealed that the mutants significantly reduced DA uptake relative to that displayed by WT HA:DAT-1. dat-1(vt21) exhibited low, but detectible, DA transport activity.Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
WBPhenotype:0002114Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
RemarkThis mutant demonstrates a stable Swip phenotype and can be rescued by reserpine pretreatment. Phenotype not lost after 3X outcrossing.Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
Rescued_by_transgeneWBTransgene00015309
Affected_byMoleculeWBMol:00002955Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
WBPhenotype:0002375Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
Remarkdat-1 lines feature a significant number of animals that revert back to relatively normal swimming behavior that we tabulated as reversion incidence (no. revertants/no. paralyzers) and reversion probability (time in reversion/time after paralysis).Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
Phenotype_not_observedWBPhenotype:0000905Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
RemarkCEP, ADE and PDE cell bodies and processes appear unaltered relative to N2 animals.Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
EQ_annotationsAnatomy_termWBbt:0005244PATO:0000460Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
WBbt:0005415PATO:0000460Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
WBbt:0006747PATO:0000460Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
WBbt:0005278PATO:0000460Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
WBPhenotype:0002114Paper_evidenceWBPaper00041219
Curator_confirmedWBPerson712
ReferenceWBPaper00041219
MethodSubstitution_allele