WormBase Tree Display for Variation: WBVar02154205

WBVar02154205 Evidence: Curator_confirmed: WBPerson1983
  Name: Public_name: ve647
    HGVSg: CHROMOSOME_II:g.6734836_6735950del
  Sequence_details: SMap: S_parent: Sequence: T14B4
    Flanking_sequences: aaccaatgctgatgaaatcaacttccacgg gatgggtagacagaagaaagattagaatta
    Mapping_target: T14B4
    Type_of_mutation: Deletion
    SeqStatus: Sequenced
  Variation_type: Engineered_allele
  Origin: Species: Caenorhabditis elegans
    Strain: WBStrain00049386
    Production_method: CRISPR_Cas9
    Status: Live
  Affects: Gene: WBGene00020500
    Transcript: T14B4.3.1 VEP_consequence: splice_acceptor_variant,splice_donor_variant,coding_sequence_variant,5_prime_UTR_variant,intron_variant
        VEP_impact: HIGH
        cDNA_position: ?-879
        CDS_position: ?-866
        Protein_position: ?-289
        Intron_number: 2-4/5
        Exon_number: 1-5/6
  Remark: Whole gene deletions made by the Rougvie, Moerman, Hutter and Sternberg labs that replace the coding sequence with a [LoxP + myo-2p::GFP::unc-54 3Prime UTR + rps-27p::neoR::unc-54 3Prime UTR + LoxP] cassette.
    Allele Description:T14B4.3(ve647[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) II.
    sgRNA1:caagagaacttgaaactccg sgRNA2: GATCCTGGTTCGACTATCGA
  Method: Engineered_allele