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WormBase Tree Display for Variation: WBVar02154248

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Name Class

WBVar02154248EvidenceCurator_confirmedWBPerson1983
NamePublic_nameve690
Sequence_detailsSMapS_parentSequenceY54H5A
Flanking_sequencescacttgcagtttccgcttgatcacccaaatcccgggtacgcgtccttctcaccgacaaac
Mapping_targetY54H5A
Type_of_mutationDeletion
SeqStatusSequenced
Variation_typeEngineered_allele
OriginSpeciesCaenorhabditis elegans
StrainWBStrain00049429
Production_methodCRISPR_Cas9
StatusLive
AffectsGeneWBGene00174448
WBGene00305827
WBGene00021900
TranscriptY54H5A.2.1
Y54H5A.6
Y54H5A.7
RemarkWhole gene deletions made by the Rougvie, Moerman, Hutter and Sternberg labs that replace the coding sequence with a [LoxP + myo-2p::GFP::unc-54 3Prime UTR + rps-27p::neoR::unc-54 3Prime UTR + LoxP] cassette.
Allele Description:Y54H5A.2(ve690[LoxP + myo-2p::GFP::unc-54 3' UTR + rps-27p::neoR::unc-54 3' UTR + LoxP]) III.
sgRNA1:ccgcttgatcacccaaatta sgRNA2: gtatacctcattcgcccacc
MethodEngineered_allele