WormBase Tree Display for Variation: WBVar02158464
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| WBVar02158464 | Possibly_affects | WBGene00009498 | |||||
|---|---|---|---|---|---|---|---|
| Description | Phenotype | WBPhenotype:0000050 | Paper_evidence | WBPaper00065094 | |||
| Curator_confirmed | WBPerson712 | ||||||
| Remark | ...embryos rarely hatched, similar to RNAi knockdown of tat-5 causing embryonic lethality (Wehman et al., 2011). | Paper_evidence | WBPaper00065094 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Variation_effect | Hypomorph_reduction_of_function | Paper_evidence | WBPaper00065094 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| WBPhenotype:0001135 | Paper_evidence | WBPaper00065094 | |||||
| Curator_confirmed | WBPerson712 | ||||||
| Remark | tat-5(D244T) mutant embryos were defective in P0 midbody remnant internalization (Figure 1B), which normally occurs by the 6-cell stage (Fazeli et al., 2016). | Paper_evidence | WBPaper00065094 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Variation_effect | Hypomorph_reduction_of_function | Paper_evidence | WBPaper00065094 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| WBPhenotype:0001145 | Paper_evidence | WBPaper00065094 | |||||
| Curator_confirmed | WBPerson712 | ||||||
| Remark | tat-5(D244T) mutant embryos were defective in second polar body internalization (Figure 1C), which normally occurs by the early 4-cell stage (Fazeli et al., 2018). | Paper_evidence | WBPaper00065094 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Variation_effect | Hypomorph_reduction_of_function | Paper_evidence | WBPaper00065094 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| WBPhenotype:0001332 | Paper_evidence | WBPaper00065094 | |||||
| Curator_confirmed | WBPerson712 | ||||||
| Remark | We found that control embryos had an average of 32 mCh::PH::CTPD puncta on their surface (Fig. 1D-E). In contrast, tat-5(D244T) mutants had an average of 18167 mCh::PH::CTPD puncta (Figure 1D, F), suggesting a >60-fold increase in EV release. These data indicate that despite being able to support fertility, TAT-5(D244T) loses its ability to regulate EV release. | Paper_evidence | WBPaper00065094 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Variation_effect | Hypomorph_reduction_of_function | Paper_evidence | WBPaper00065094 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Phenotype_not_observed | WBPhenotype:0000688 | Paper_evidence | WBPaper00065094 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Remark | We found that tat-5(D244T) mutants were fertile, laying an average of 7743 embryos and 118 oocytes (Fig. 1A). | Paper_evidence | WBPaper00065094 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Variation_effect | Hypomorph_reduction_of_function | Paper_evidence | WBPaper00065094 | ||||
| Curator_confirmed | WBPerson712 | ||||||
| Reference | WBPaper00065094 |
