WormBase Tree Display for Expr_pattern: Expr1896

Expr1896 Expression_of: Gene: WBGene00004202
    Reflects_endogenous_expression_of: WBGene00004202
  Expression_data: Anatomy_term: WBbt:0004054 Certain
      WBbt:0004056 Certain
      WBbt:0004145 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0004147 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0004149 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0004151 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0004153 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0004155 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0004409 Certain
      WBbt:0004410 Certain
      WBbt:0004448 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0004477 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0004874 Certain
      WBbt:0004875 Certain
      WBbt:0004876 Certain
      WBbt:0004890 Certain
      WBbt:0005300 Certain
      WBbt:0005757 Certain
        Partial
      WBbt:0005813 Certain
      WBbt:0006751 Certain
      WBbt:0006759 Certain
      WBbt:0006762 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0006763 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0006764 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0006765 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0006766 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0006767 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0006768 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0006776 Certain
      WBbt:0006777 Certain
      WBbt:0006778 Certain
      WBbt:0006779 Certain
      WBbt:0006983 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0006984 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0006987 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0006988 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0006989 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0006990 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0006991 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0007252 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0007253 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0007254 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0007255 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0007256 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0007260 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0007264 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0007265 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0007266 Uncertain
        Remark: Expressed in developing vulva.
      WBbt:0007274 Certain
      WBbt:0007276 Certain
      WBbt:0007279 Certain
      WBbt:0007281 Certain
      WBbt:0008112 Certain
      WBbt:0008117 Certain
      WBbt:0008121 Certain
      WBbt:0008125 Certain
      WBbt:0008129 Certain
      WBbt:0008133 Certain
  Type: Reporter_gene
  Pattern: The pry-1 reporter gene is widely expressed throughout development. Expression starts halfway through embryogenesis and is mainly localized to the ventral and lateral hypodermal cells. At the early L1 stage, pry-1 is expressed at high levels in the lateral hypodermal cells (or seam cells) V5 and V6 and in the Q neuroblasts QL and QR. pry-1 is also expressed in the ventral hypodermal (P) cells P7/8 to P11/12, body wall muscle cells, and neurons in the head, the tail, and the ventral nerve cord. No differences in pry-1 expression levels between QL and QR was observed, but this may be a result of PRY-1 GFP overexpression. At the end of the L1 stage, pry-1 is expressed at high levels in all seam cells. Expression was also observed in the QL and QR daughter cells. At later larval stages, pry-1 is expressed at high levels throughout the animal, including hypodermal cells, body wall muscle cells, and many neurons in the ventral nerve cord and head and tail ganglia. In addition, pry-1 is expressed in the vulva precursor (Pn.p) cells and in the developing vulva and male tail.
  Picture: WBPicture0000008438
  Remark: The expression pattern of this construct was similar in independent transgenic lines, but the subcellular localization of the PRY-1 GFP fusion protein differed, ranging from localization at the plasma membrane and in cytoplasmic dots to diffuse cytoplasmic and nuclear staining. This difference in subcellular localization may be a consequence of variations in expression levels of the fusion protein in different transgenic lines. For ease of cell identification, a transgenic line showing diffuse cytoplasmic and nuclear staining was selected. This transgene fully rescued the lethality, the multivulva phenotype, and the QR.d migration defect of pry-1(mu38 and nc1). This suggests that the PRY-1 GFP fusion protein is functional and is correctly expressed in cells in which PRY-1 is essential.
  Reference: WBPaper00005278
  Transgene: WBTransgene00000302