WormBase Tree Display for Expr_pattern: Expr2247

Expr2247 Expression_of: Gene: WBGene00003735
    Reflects_endogenous_expression_of: WBGene00003735
  Expression_data: Anatomy_term: WBbt:0004510 Certain
      WBbt:0004511 Certain
      WBbt:0005451 Certain
      WBbt:0005753 Certain
      WBbt:0005767 Certain
      WBbt:0006748 Certain
    GO_term: GO:0031090
  Subcellular_localization: NHX-8 appeared to be associated with intracellular membranes. The NHX-8::GFP protein was distributed in the seam cells in a vesicular pattern that excluded the nucleus, with some underlying signal contributed by weakly fluorescent hypodermal syncitia.
  Type: Reporter_gene
  Pattern: In general, the expression patterns found for the translational fusion constructs were similar to those reported above for the transcriptional fusions. NHX-8::GFP was expressed as expected in the seam cells but was also expressed in the pharyngeal muscles, the pharyngeal-intestinal and intestinal-rectal valve cells, and in cells of the vulva. Expression in these additional cells may have been a result of promoter or enhancer elements in the first intron of the nhx-8 gene that are not present in the transcriptional fusion construct. Alternatively, the shorter promoter used in the translational reporter construct (1.5 versus 4 kb) may lack sequences that normally repress expression in these cells. See Expr2237 for expression pattern observed with transcriptional fusion construct.
  Picture: WBPicture0000008208
  Reference: WBPaper00005421
  Transgene: WBTransgene00027959