WormBase Tree Display for Expr_pattern: Expr2451
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| Expr2451 | Expression_of | Gene | WBGene00004333 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004333 | |||
| Expression_data | Anatomy_term | WBbt:0005175 | Certain | |
| GO_term | GO:0000794 | |||
| Subcellular_localization | In the gonad, REC-8 was localized as small grains all over the nuclei of the mitotic zone, whereas in the transition zone and in pachytene nuclei, it assembled into short and long threads, respectively. In diakinesis, REC-8 was localized along the axes of chromosomes. From metaphase I through to metaphase II REC-8 signals appeared to become progressively less intense or smaller. In metaphase I, both the poleward and equatorial axes of bivalents were decorated with REC-8 whereas in early anaphase I chromosomes, the length of REC-8 immunosignals seemed to be restricted to the original poleward portion of metaphase I chromosomes. In late anaphase I, individual chromosomes could no longer be discriminated and REC-8 spots were visible within the highly condensed DAPI-positive masses of the two half-nuclei. In metaphase II short tracts of REC-8 were observed in the equatorial plane. | |||
| Type | Antibody | Polyclonal rabbit or rat antibodies. Indirect immunostaining with rabbit or rat antiserum against a 350 amino acid fragment. | ||
| Pattern | The protein was absent from somatic cells. Expressed in the gonad. | |||
| Picture | WBPicture0000008419 | |||
| WBPicture0000008420 | ||||
| WBPicture0000008421 | ||||
| Remark | No immunofluorescence was detected after rec-8 RNAi, i.e., in the gonads of F1 worms from embryos laid by animals 24 h or later after dsRNA injection. Also the staining with preimmune serum was negative. | |||
| Reference | WBPaper00004722 | |||
| Antibody_info | WBAntibody00000373 | |||
| WBAntibody00000374 |
