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| WBPicture0000008420 | Description | Figure 5. REC-8 immunostaining pattern throughout gonadal mitosis and meiosis. (A) Grains in nuclei of the mitotic zone. (B) Formation of short threads (arrows) in the transition zone (leptotene/zygotene). (C) REC-8 delineates synaptonemal complexes (SCs) in pachytene. In D and E immunostaining is shown on top and DAPI at the bottom, in F immunostaining is on the left and DAPI on the right. Diakinesis: Labeling of chromosomal axes in bivalents (D) and spo-11 univalents (E). Insert in D shows an enlarged DAPI-stained bivalent (arrows) with the inverted image of the REC-8 axes superimposed. (F) From top to bottom: Metaphase I (side view, two bivalents in focus, arrows denote equator), early anaphase I (top view of half-nucleus; the other half is in a different focal plane underneath), late anaphase I (separating nuclei out of alignment due to preparation), metaphase II (side view, three chromosomes in the focal plane; arrows denote equator, polar body outside the area shown). Bar in C represents 10 μm in A-C; bar in D, 5 μm in D-F. | |||
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| Name | F5.large.jpg | ||||
| Depict | Expr_pattern | Expr2451 | |||
| Anatomy | WBbt:0005175 | ||||
| Cellular_component | GO:0000790 | ||||
| GO:0000794 | |||||
| GO:0000795 | |||||
| GO:0005634 | |||||
| Acknowledgment | Template | WormBase wishes to thank <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |||
| Publication_year | 2001 | ||||
| Article_URL | DOI | id | 10.1101/gad.192701 | ||
| Journal_URL | Genes&Development | ||||
| Publisher_URL | ColdSpringHarborLabPress | ||||
| Reference | WBPaper00004722 |
