WormBase Tree Display for Expr_pattern: Expr2779
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| Expr2779 | Expression_of | Gene | WBGene00001827 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00001827 | ||
| Expression_data | GO_term | GO:0005634 | |
| GO:0005739 | |||
| Subcellular_localization | CeHCF showed a distinctly different and complex localisation whereby the typical pattern in ~75% cells showed a cytoplasmic threadlike pattern combined with a speckled distribution in the nucleus. To identify the very distinct cytoplasmic accumulation of CeHCF in mammalian cells, CeHCF distribution was examined in combination with a series of markers for different cytoplasmic compartments. The punctate localisation of CeHCF represented very specific and frequently quantitative colocalisation with mitochondria. This was observed with several independent mitochondrial markers for mitotracker, a mitochondrial specific dye. More diffuse cytoplasmic staining could sometimes be observed in addition to the cytoplasmic mitochondrial localisation, and in a significant fraction of cells (~25%) there was very little nuclear accumulation. | ||
| Remark | Type: mammalian cell transfection. Cells were transiently transfected with expression vectors for HCF-1 proteins containing an epitope tag at the N-terminus to facilitate detection and analysed by immunofluorescence. | ||
| Reference | WBPaper00006222 |
