WormBase Tree Display for Expr_pattern: Expr2780

Expr2780 Expression_of: Gene: WBGene00001827
    Reflects_endogenous_expression_of: WBGene00001827
  Expression_data: GO_term: GO:0005634
      GO:0005739
      GO:0005737
  Subcellular_localization: CeHCF-GFP showed strong mitochondrial and nuclear localisation in mammalian cells, both live and after fixation. CeHCFDC1-GFP accumulated diffusely in the cytoplasm with little if any nuclear or mitochondrial localisation.
  Remark: To address the possibility that the accumulation pattern of CeHCF in mammalian cells was the result of overexpression in transient transfection assay, a HeLa cell line stably expressing CeHCF-GFP was established . The cells expressed the protein in lower but readily detectable amounts. Strikingly, when stably expressed the CeHCF-GFP protein accumulated mainly in mitochondria, showing very little if any nuclear accumulation. These results suggested that the CeHCF is targeted very effectively to mitochondria in mammalian cells, with nuclear accumulation probably resulting from higher expression levels (for example saturating the mitochondrial association).
    Type: mammalian cell transfection. A mammalian expression vector for CeHCF-GFP was constructed. A mutant lacking the C1 tail was also constructed in this context (CeHCFDC1-GFP).
  Reference: WBPaper00006222