WormBase Tree Display for Expr_pattern: Expr9296
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| Expr9296 | Expression_of | Gene | WBGene00010251 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00010251 | ||
| Expression_data | GO_term | GO:0005634 | |
| GO:0030139 | |||
| GO:0045177 | |||
| Subcellular_localization | In worms expressing a GFP::STA-2 fusion reporter in the epidermis (pcol-12::GFP::STA-2), a fluorescent signal was clearly seen in the nucleus but also in bands underneath the cuticle and in some vesicles. Equivalent patterns were also obtained when STA-2 was tagged with DsRed2 or mCherry and when the fusion protein was expressed under the control of the sta-2 promoter (psta- 2::GFP::STA-2). As the different chimeric proteins were functional, the localization seen with these reporter proteins is likely to reflect the distribution of the endogenous protein. Using the mCherry::STA-2 construct,we found that the fluorescent signal colocalized with a clathrin heavy-chain CHC-1::GFP reporter protein, a specific marker of newly formed endocytic vesicles (Greener et al., 2001; Sato et al., 2009). | ||
| Type | Reporter_gene | ||
| Reference | WBPaper00038424 | ||
| Transgene | WBTransgene00031056 |
