WormBase Tree Display for Expr_pattern: Expr988
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| Expr988 | Expression_of | Gene | WBGene00002994 |
|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00002994 | ||
| Expression_data | Life_stage | WBls:0000002 | |
| GO_term | GO:0005819 | ||
| GO:0005737 | |||
| GO:0016020 | |||
| GO:0000793 | |||
| GO:0005828 | |||
| Subcellular_localization | cytoplasmic, membrane-associated or chromosome associated (nuclei). | ||
| Type | Antibody | Mouse monoclonal antibody to LIN-5 recombinant protein expressed in E. coli. | |
| Pattern | LIN-5 was present at the meiotic spindles in the fertilized egg during meiosis I and II. Staining associated with the maternal pronucleus disappeared after completion of meiosis and LIN-5 became localized at the duplicated centrosomes that adjoined the paternal pronucleus. LIN-5 remained localized at the centrosomes until completion of the first mitotic division. In all subsequent mitoses, LIN-5 was associated with the centrosomes and detectable after separation of the centrosomes in prophase until decondensation of chromosomes in telophase. LIN-5 was also diffusely present in the cytoplasm and at two other locations; LIN-5 appeared microtubule-associated in cells with metaphase-aligned chromosomes. This localization was limited to the region between the poles and did not overlap with DNA staining. Therefore, it appeared to reflect specific association of LIN-5 with kinetochore microtubules. LIN-5 was diffusely present at the cell cortex in most embryonic cells from the two-cell stage onward. During the larval stages, LIN-5 localized to the centrosomes from prophase to telophase and to the cell periphery in late mitosis. In adult hermaphrodites, mitosis is restricted to germ presursor cells in the distal ends of the gonad. LIN-5 was detected at the centrosomes of such cells and, in addition, diffuse cytoplasmic LIN-5 staining was detected in oocytes. Strong membrane-associated staining was detected in the gonad at all stages of development. | ||
| Picture | WBPicture0000008927 | ||
| Reference | WBPaper00003888 | ||
| Antibody_info | WBAntibody00000263 | ||
| WBAntibody00000264 | |||
| WBAntibody00000265 |
