WormBase Tree Display for Antibody: WBAntibody00000264
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| WBAntibody00000264 | Summary | Mouse monoclonal antibody to LIN-5 recombinant protein expressed in E. coli. Fusion of splenocytes with myeloma Sp2 cells and selection of hybridomas was performed according to standard procedures. Antibodies produced by clone hel-2 are of the IgG1 isotype. Light chains are of the k-type. | ||
|---|---|---|---|---|
| Public_name | [cgc3888]:lin-5_b | |||
| Other_name | hel-2 is the name of hybridoma. | |||
| Gene | WBGene00002994 | |||
| Isolation | Location | SV | ||
| Clonality | Monoclonal | |||
| Antigen | Protein | A 3' fragment of the lin-5 cDNA (1,138 bp SalIBamHI) was cloned into the pET19b expression vector (Novagen) and expressed in Escherichia coli. The purified His-tagged LIN-5 COOH-terminal fragment was injected into mice. | ||
| Animal | Mouse | |||
| Expr_pattern | Expr988 | |||
| Interactor | WBInteraction000501430 | |||
| Reference | WBPaper00003888 | |||
| WBPaper00005878 | ||||
| WBPaper00042433 |
