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WormBase Tree Display for Construct: WBCnstr00001622

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Name Class

WBCnstr00001622Summary[unc-80::gfp]
Driven_by_geneWBGene00006812
Fusion_reporterGFP
Type_of_constructTranscriptional_fusion
Construction_summaryThe unc-80 promoter was amplified from genomic DNA with the following primers: U80A2 5' -GATATCCGCATATTGAGAGCTTTAG-3' and U80B 5 -CCTTTACTCATTTTTTCTACCGGTACcaatggcactgaatcacattcttc-3'. GFP sequence was amplified with primers U80C 5' -GTACCGGTAGAAAAAATGAGTAAAGG-3' and U80D 5' -AAGGGCCCGTACGGCCGACTAGTAGG-3'. The two fragments were mixed together and then PCR was performed with the outside primers, U80A and U80D. The PCR fragment was injected into lin-15(n765ts) mutants at 5 ng/ml. Three lines were obtained that had similar expression, and the analyzed array is oxEx839. --precise ends.
Used_forTransgene_constructWBTransgene00001633
ReferenceWBPaper00031000