WormBase Tree Display for Construct: WBCnstr00001622
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WBCnstr00001622 | Summary | [unc-80::gfp] | |
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Driven_by_gene | WBGene00006812 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Transcriptional_fusion | ||
Construction_summary | The unc-80 promoter was amplified from genomic DNA with the following primers: U80A2 5' -GATATCCGCATATTGAGAGCTTTAG-3' and U80B 5 -CCTTTACTCATTTTTTCTACCGGTACcaatggcactgaatcacattcttc-3'. GFP sequence was amplified with primers U80C 5' -GTACCGGTAGAAAAAATGAGTAAAGG-3' and U80D 5' -AAGGGCCCGTACGGCCGACTAGTAGG-3'. The two fragments were mixed together and then PCR was performed with the outside primers, U80A and U80D. The PCR fragment was injected into lin-15(n765ts) mutants at 5 ng/ml. Three lines were obtained that had similar expression, and the analyzed array is oxEx839. --precise ends. | ||
Used_for | Transgene_construct | WBTransgene00001633 | |
Reference | WBPaper00031000 |