WormBase Tree Display for Construct: WBCnstr00007234
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| WBCnstr00007234 | Public_name | GB110 | |
|---|---|---|---|
| Summary | [kal-1; Fe65::GFP; rol-6(su1006)] | ||
| Driven_by_gene | WBGene00002181 | ||
| Gene | WBGene00002181 | ||
| Fusion_reporter | GFP | ||
| Construction_summary | Clone = GB110. | Plasmid CeKAL was obtained by replacing GFP coding sequence in plasmid GB102 with that of kal-1 cDNA sequnce from the ATG to the stop codon. GB102 was generated through directional cloning of the PCR amplified kal-1 regulatory region fragment of 4.3 kb into the SphI and BamHI sites of vectors pPD95.75. Plasmid GB110(Fe65::GFP) and plasmid pRF4, were co-injected as selectable markers (Rugarli et al., 2002). | ||
| Used_for | Transgene_construct | WBTransgene00007421 | |
| Reference | WBPaper00005138 | ||
| WBPaper00036132 |
