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WormBase Tree Display for Transgene: WBTransgene00007421

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Name Class

WBTransgene00007421Public_namegbEx13
Summary[kal-1; Fe65::GFP; rol-6(su1006)]
ConstructionConstructWBCnstr00007234
CoinjectionWBCnstr00004720
Construction_summaryClone = GB110.
Plasmid CeKAL was obtained by replacing GFP coding sequence in plasmid GB102 with that of kal-1 cDNA sequnce from the ATG to the stop codon. GB102 was generated through directional cloning of the PCR amplified kal-1 regulatory region fragment of 4.3 kb into the SphI and BamHI sites of vectors pPD95.75. Plasmid GB110(Fe65::GFP) and plasmid pRF4, were co-injected as selectable markers (Rugarli et al., 2002).
Genetic_informationExtrachromosomal
ReferenceWBPaper00005138
WBPaper00036132
SpeciesCaenorhabditis elegans