WormBase Tree Display for Construct: WBCnstr00010750
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| WBCnstr00010750 | Other_name | Expr2283_Ex | |
|---|---|---|---|
| Summary | [unc-5::unc-5-gfp] | ||
| Driven_by_gene | WBGene00006745 | ||
| Gene | WBGene00006745 | ||
| Fusion_reporter | GFP | ||
| Type_of_construct | Translational_fusion | ||
| Construction_summary | [unc-5::unc-5-gfp] and [unc-5::unc-5-HA] functional translational fusion. The construct pU5 was made containing 4.5 kb of DNA sequence 5 to exon 2, the entire unc-5 cDNA from the start of exon 2 to exon 9, and 1 kb of 3' UTR. Exon 1 was not included since it appears to be dispensable for function. This construct has an initiation methionine (defined here as residue number 1) in exon 2 of unc-5 and encodes an UNC-5 protein of 919 amino acids. Tagged versions were made as follows: pU5::GFP was made by inserting GFP from pPD95.79 into a BsaBI site. This results in an in-frame fusion with GFP after the codon for F909 of UNC-5. pU5::HA was made by cloning 3 in-frame HA tags on a BglII fragment after the codon for D889 of UNC-5. --precise ends. | ||
| Clone | pPD95.79 | ||
| Used_for | Transgene_construct | WBTransgene00027981 | |
| Reference | WBPaper00005582 |
