WormBase Tree Display for Construct: WBCnstr00017692
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| WBCnstr00017692 | Summary | [Punc-47::sng-1::GFP; unc-119(+)] | |
|---|---|---|---|
| Driven_by_gene | WBGene00006783 | ||
| Gene | WBGene00004912 | ||
| Fusion_reporter | GFP | ||
| Construction_summary | Three-fragment Multisite Gateway vectors were used for generating this construct. PENTRY4-1 was used as the slot 1 promoter entry vector; the promoter fragment does not include the initiating methionine codon (ATG). PENTRY1-2 was used as the slot 2 ORF entry vector, which has an ATG immediately following the att site at the beginning of the ORF; they do not have a stop codon at the 3' end. PENTRY2-3 was used for the slot 3 C-terminal tag and 3'UTR entry vector, slot 3 clones was GFP-unc-54 3'UTR. The destination vectors was pCFJ201 for MosSCI on chromosome IV. | ||
| Used_for | Transgene_construct | WBTransgene00018307 | |
| Reference | WBPaper00042286 |
