WormBase Tree Display for Construct: WBCnstr00019421
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WBCnstr00019421 | Summary | [ser-6::gfp] | |
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Driven_by_gene | WBGene00021897 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Transcriptional_fusion | ||
Construction_summary | The transcriptional reporter fusion gene ser-6::gfp was generated using the fusion PCR method as described elsewhere (Hobert, 2002). The region corresponding to 5.0-kb upstream and a part of exon 1 of ser-6 gene was amplified with the primers Y54fusionA (5'-GTTAAGCTCCTCGAACTTTCGG-3') and Y54fusionB (5'-AGTCGACCTGCAGGCATGCAAGCTGCCCAGCGTCAGTGATA GC-3') by LA Taq (TaKaRa) using genomic DNA as the template. The resulting PCR product was fused to 2-1876 of pPD95.75. | ||
Used_for | Transgene_construct | WBTransgene00031584 | |
Reference | WBPaper00044729 |