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WormBase Tree Display for Construct: WBCnstr00019421

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Name Class

WBCnstr00019421Summary[ser-6::gfp]
Driven_by_geneWBGene00021897
Fusion_reporterGFP
Type_of_constructTranscriptional_fusion
Construction_summaryThe transcriptional reporter fusion gene ser-6::gfp was generated using the fusion PCR method as described elsewhere (Hobert, 2002). The region corresponding to 5.0-kb upstream and a part of exon 1 of ser-6 gene was amplified with the primers Y54fusionA (5'-GTTAAGCTCCTCGAACTTTCGG-3') and Y54fusionB (5'-AGTCGACCTGCAGGCATGCAAGCTGCCCAGCGTCAGTGATA GC-3') by LA Taq (TaKaRa) using genomic DNA as the template. The resulting PCR product was fused to 2-1876 of pPD95.75.
Used_forTransgene_constructWBTransgene00031584
ReferenceWBPaper00044729