WormBase Tree Display for Construct: WBCnstr00020378
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WBCnstr00020378 | Public_name | pMR753 |
---|---|---|
Summary | ATGL-1::HA | |
Gene | WBGene00015484 | |
Purification_tag | HA-tag | |
Type_of_construct | Cterminal_translational_fusion | |
Construction_summary | Two PCR reactions were performed using the pMR751 plasmid as a template; one with rr1087-rr1088 and one with rr1089-rr1090 primers (sequence available upon request). Each resulting fragment was digested with SacI-NotI and they were ligated together to generate an ATGL-1::HA construct (pMR753). | |
Reference | WBPaper00032396 | |
Laboratory | MR |