WormBase Tree Display for Construct: WBCnstr00022342
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WBCnstr00022342 | Summary | [pclec-60::GFP] | |
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Driven_by_gene | WBGene00014046 | ||
Fusion_reporter | GFP | ||
Type_of_construct | Transcriptional_fusion | ||
Construction_summary | [pclec-60::GFP] transcriptional fusion. PCR was used to amplify 1,665 bp of DNA upstream of theclec-60 start site. Splicing by overlapping extension PCR wasused as described to generate a clec-60 promoter-GFP-unc-543'UTR fusion. Fwd primer: ACGGGCAAGTTATTGGAGAG. Rev primer: ACACGGTATTGAATCCACGA. | ||
Used_for | Transgene_construct | WBTransgene00031886 | |
Reference | WBPaper00032331 |