WormBase Tree Display for Construct: WBCnstr00039800
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| WBCnstr00039800 | Public_name | pEL228 |
|---|---|---|
| Summary | [Pmex-6::mCherry::FKBP12; LoxP] | |
| Driven_by_gene | WBGene00003231 | |
| Gene | WBGene00003231 | |
| Fusion_reporter | mCherry | |
| Other_reporter | codon optimized human FKBP12 | |
| Recombination_site | LoxP | |
| Construction_summary | We digested pEL226 with SpeI and NotI and subsequently column purified the DNA. We amplified ~575 bp 5' and 3' homology region of the C-terminus of mex-6 from genomic DNA(3' homology region includes the stop codon of mex-6 exon7, PAM(mex-6.1) was mutated to NCA) with primers carrying homology tails (Table 1) to the unique SpeI and NotI sites of pEL226. PCR products were subsequently column purified. All four fragments (PCR products plus pEL226 fragments) were fused together via Gibson assembly(pEL228). | |
| Reference | WBPaper00054190 |
