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WormBase Tree Display for Construct: WBCnstr00041293

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Name Class

WBCnstr00041293Summary[sad-1::RFP]
Driven_by_geneWBGene00004719
GeneWBGene00004719
Fusion_reporterRFP
Construction_summary[sad-1::RFP]. sad-1 included isoform. sad-1 minigenes were created using the following primers: Forward 5' GATAAAACTGAAACAAC TTCTGC and Reverse 5' GGGGTTGGCGATTTGTATGAGaTAGC. Restriction sites were appended to both the forward primer (XhoI) and reverse (NotI) primers to facilitate cloning into a Gateway- compatible vector as previously described (Norris et al., 2014). The reporter was then cloned down- stream of a pan-neuronal rgef-1 promoter, as endogenous sad-1 has been detected broadly throughout the nervous system
Used_forTransgene_constructWBTransgene00032338
ReferenceWBPaper00057064