WormBase Tree Display for Construct: WBCnstr00041293
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WBCnstr00041293 | Summary | [sad-1::RFP] | |
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Driven_by_gene | WBGene00004719 | ||
Gene | WBGene00004719 | ||
Fusion_reporter | RFP | ||
Construction_summary | [sad-1::RFP]. sad-1 included isoform. sad-1 minigenes were created using the following primers: Forward 5' GATAAAACTGAAACAAC TTCTGC and Reverse 5' GGGGTTGGCGATTTGTATGAGaTAGC. Restriction sites were appended to both the forward primer (XhoI) and reverse (NotI) primers to facilitate cloning into a Gateway- compatible vector as previously described (Norris et al., 2014). The reporter was then cloned down- stream of a pan-neuronal rgef-1 promoter, as endogenous sad-1 has been detected broadly throughout the nervous system | ||
Used_for | Transgene_construct | WBTransgene00032338 | |
Reference | WBPaper00057064 |