WormBase Tree Display for Gene: WBGene00016625
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WBGene00016625 | SMap | S_parent | Sequence | C44B7 | |||
---|---|---|---|---|---|---|---|
Identity (6) | |||||||
Gene_info | Biotype | SO:0001217 | |||||
Gene_class | aff | ||||||
Reference_allele | WBVar00266475 | Paper_evidence | WBPaper00029363 | ||||
Allele (26) | |||||||
Strain | WBStrain00003881 | ||||||
WBStrain00000197 | |||||||
WBStrain00003884 | |||||||
WBStrain00003885 | |||||||
WBStrain00003886 | |||||||
WBStrain00047003 | |||||||
RNASeq_FPKM (74) | |||||||
GO_annotation | 00020506 | ||||||
00020507 | |||||||
00020508 | |||||||
00020509 | |||||||
00020510 | |||||||
00020511 | |||||||
00020512 | |||||||
00020513 | |||||||
00020514 | |||||||
00020515 | |||||||
Ortholog (25) | |||||||
Paralog | WBGene00001159 | Caenorhabditis elegans | From_analysis | TreeFam | |||
Panther | |||||||
WormBase-Compara | |||||||
WBGene00023420 | Caenorhabditis elegans | From_analysis | TreeFam | ||||
Panther | |||||||
WormBase-Compara | |||||||
Structured_description | Concise_description | aff-1 encodes a cell-surface protein required in late L4 larvae forvarious cell fusions, of which at least one (AC-utse) does not requireEFF-1; in vulval development, AFF-1 is required for fusion of the anchorcell (AC) with the utse syncytium, of A cells to one another, and of Dcells to one another; AFF-1 is also required for lateral seam cellfusions; since aff-1 mutants fail to mix AC with utse cytoplasm, AFF-1is probably required to begin cell fusion by forming intercellularpores; AFF-1 has no obvious non-nematode orthologs, but is paralogous toEFF-1 and C26D10.7; AFF-1 is predicted to be largely extracellular, witheight conserved predicted disulfide bonds in its ectodomain and a singleC-terminal transmembrane domain; AFF-1 is expressed in the AC, beginningaround the AC's invasion of basement membrane in mid-L3 larvae, andcontinuing until the AC's fusion to utse; AFF-1 is also expressed inutse, beginning in L4 larvae, after which AC-utse fusion immediatelyensues; in both AC and utse, AFF-1 expression requires FOS-1, and theaff-1 promoter has predicted FOS-1 binding sites; other sites of AFF-1expression include embryonic hyp5 cells, pharyngeal muscles (Pm3 andPm5), head and tail neurons, sheath cells of chemosensory neurons, andmale tail neurons; subcellularly, AFF-1::GFP localizes to plasmamembrane and (undefined) intracellular organelles; generally, AFF-1 isexpressed in cells whose fusion does not require EFF-1, which mayexplain why AFF-1 and EFF-1 are redundantly required for viability; heatshock-driven overexpression of AFF-1, even in an eff-1 null mutantbackground, can induce ectopic cell fusion, and heterologous AFF-1 caninduce insect Sf9 cells to fuse in vitro. | Paper_evidence | WBPaper00029363 | |||
Curator_confirmed | WBPerson567 | ||||||
Date_last_updated | 04 Jun 2007 00:00:00 | ||||||
Automated_description | Involved in regulation of egg-laying behavior and syncytium formation by plasma membrane fusion. Located in plasma membrane. Expressed in anchor cell; egg-laying apparatus; epithelial cell; neurons; and pharyngeal muscle cell. | Paper_evidence | WBPaper00065943 | ||||
Curator_confirmed | WBPerson324 | ||||||
WBPerson37462 | |||||||
Inferred_automatically | This description was generated automatically by a script based on data from the WS291 version of WormBase | ||||||
Date_last_updated | 29 Nov 2023 00:00:00 | ||||||
Molecular_info | Corresponding_CDS | C44B7.3 | |||||
Corresponding_CDS_history | C44B7.3:wp88 | ||||||
C44B7.3:wp179 | |||||||
Corresponding_transcript | C44B7.3.1 | ||||||
Associated_feature | WBsf717693 | ||||||
WBsf988281 | |||||||
WBsf988282 | |||||||
WBsf988283 | |||||||
WBsf1012448 | |||||||
WBsf223240 | |||||||
Experimental_info | RNAi_result (11) | ||||||
Expr_pattern (13) | |||||||
Drives_construct | WBCnstr00000620 | ||||||
WBCnstr00012152 | |||||||
WBCnstr00012153 | |||||||
WBCnstr00012510 | |||||||
WBCnstr00013132 | |||||||
WBCnstr00027928 | |||||||
WBCnstr00040834 | |||||||
WBCnstr00040835 | |||||||
Construct_product | WBCnstr00008101 | ||||||
WBCnstr00012152 | |||||||
WBCnstr00012153 | |||||||
WBCnstr00013132 | |||||||
WBCnstr00015967 | |||||||
WBCnstr00027928 | |||||||
WBCnstr00040835 | |||||||
Microarray_results (19) | |||||||
Expression_cluster (137) | |||||||
Interaction | WBInteraction000188024 | ||||||
WBInteraction000365196 | |||||||
WBInteraction000502906 | |||||||
WBInteraction000502907 | |||||||
WBInteraction000503580 | |||||||
WBInteraction000520686 | |||||||
WBInteraction000520687 | |||||||
Map_info | Map | II | Position | 0.125694 | Error | 0.001371 | |
Positive | Positive_clone | C44B7 | Inferred_automatically | From sequence, transcript, pseudogene data | |||
Pseudo_map_position | |||||||
Reference (37) | |||||||
Remark | Map position created from combination of previous interpolated map position (based on known location of sequence) and allele information. Therefore this is not a genetic map position based on recombination frequencies or genetic experiments. This was done on advice of the CGC. | CGC_data_submission | |||||
Method | Gene |