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WBPicture0000008986 Description: Figure 1. GFP-BAF-1 protein expression. (A) Schematic view of the baf-1- gfp construct used for generating transgenic strains. It includes the baf-1 promoter driving the gfp gene fused to the baf-1 ORF followed by the baf-1 3' untranslated region. (B-D) A stronger GFP-BAF-1 signal was detected at the nuclear periphery, whereas weaker signals were detected in the nucleoplasm and cytoplasm. GFP-BAF-1 was detected in the gonad (arrowhead in B) in young embryos (arrows in B) and in late embryos (not depicted), in L1 larvae (C), and in adult worms (arrow in D points toward the vulva). (E and F) DIC, left; GFP fluorescence, right. Although GFP-BAF-1 was localized normally at the nuclear periphery of control embryos (L4440; E), it was mislocalized in lmn-1(RNAi) embryos (arrowheads in F), where the GFP-BAF-1 was also found associated with anaphase chromatin bridges (arrow in F). The image in B was obtained with a confocal microscope, whereas images in C-F were viewed with an Axioplan II microscope (Carl Zeiss MicroImaging, Inc.). Bars, 10 um.
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  Crop: Crop_picture: WBPicture0000008987
  Acknowledgment: Template: WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. The user must not use the Work for commercial purposes. If the user shares, alters, transforms, or builds upon the Work, the user may distribute the resulting work only under the same license the user received from <Publisher_URL>.
    Publication_year: 2007
    Article_URL: DOI id 10.1083/jcb.200704049
    Journal_URL: TheJournalofCellBiology
    Publisher_URL: TheRockefellerUniversityPress
  Reference: WBPaper00030942