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WBPicture0000009597DescriptionFigure 6. RPM-1::GFP Is Expressed in the Nervous System and Localized to Presynaptic Terminal. (A) Endogenous RPM-1 expression in the nerve ring (arrow) of a wild-type animal.(B-D) Expression of RPM-1::GFP (juIs58), visualized by anti-GFP antibodies, in the nerve ring (arrow, [B]), the dorsal and subdorsal cords (arrows, [C]), and the ventral and subventral cords (arrows, [D]). Arrowhead, weak GFP expression in a ventral cord neuron cell body, excluding nuclei.(E and F) RPM-1 is localized to presynaptic terminals but is not associated with synaptic vesicles. (E) shows a confocal image of the dorsal cord of juIs58 animals costained with anti-GFP (green) and anti-SNT-1 antibodies (red). (F) shows GABAergic NMJs of transgenic animals carrying an extrachromosomal array of [Punc-30SNB-1::GFP; Punc-25RPM-1; pRF4] that are costained for RPM-1 (red) and SNB-1::GFP (green).Scale bars, 10 μm.
NameF6.jpg
DepictExpr_patternExpr1057
Expr1058
AnatomyWBbt:0005300
WBbt:0006749
Cellular_componentGO:0043025
GO:0045202
AcknowledgmentTemplateReprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
Publication_year2000
Article_URLDOIid10.1016/S0896-6273(00)81167-8
Journal_URLNeuron
Publisher_URLElsevier
ReferenceWBPaper00004146