WormBase Tree Display for Expr_pattern: Expr1057
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| Expr1057 | Expression_of | Gene | WBGene00004457 | |
|---|---|---|---|---|
| Reflects_endogenous_expression_of | WBGene00004457 | |||
| Expression_data | Anatomy_term | WBbt:0006749 | Certain | |
| GO_term | GO:0043025 | |||
| Subcellular_localization | RPM-1::GFP expression was occasionally found in some neuronal cell bodies but was excluded from nuclei. | |||
| Type | Reporter_gene | |||
| Pattern | In transgenic animals that overexpressed the functional RPM-1::GFP, both the anti-RPM-1 and anti-GFP antibodies gave identical patterns and showed that RPM-1::GFP was localized in distinct subcellular regions of the nervous system. Strong RPM-1::GFP expression was seen in the nerve ring and nerve cords but not in commissural processes that do not form synapses. Costained the transgenic animals with anti-SNT-1 antibodies and anti-GFP antibodies and analysis of confocal images revealed that RPM-1::GFP was localized adjacent to SNT-1 in presynaptic terminals. Coexpression of RPM-1 and SNB-1::GFP in the GABAergic DD and VD neurons specifically found that RPM-1 and SNB-1::GFP were present in nonoverlapping regions at individual GABAergic NMJs. | |||
| Picture | WBPicture0000009597 | |||
| Reference | WBPaper00004146 | |||
| Transgene | WBTransgene00027318 |
