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| WBPicture0000010699 | Description | Figure 3. Expression and cell-nonautonomous activity of bam-2. (A) Expression and rescue ability of bam-2 promoter derivatives driving a full-length bam-2 cDNA::gfp fusion gene. bam-2 expression from the VulF vulval cell rescues bam-2 VC neuron-branching defects. (B to D) Expression pattern of bam-2 promoter derivatives (ventral views). (B) bam-2 promoter (~600 bp) exhibits activity in a restricted subset of neurons in the ventral nerve cord and head and tail ganglia. (C) bam-2 upstream sequence (~5 kb) exhibits additional promoter activity in cells of the somatic gonad. The position of the vulva in (B) and (C) is marked by arrowheads. (D) A fusion of a ~2.4-kb distal bam-2 upstream sequence and the 600-bp proximal element exhibits promoter activity in additional longitudinal PLN axon tracts (asterisks) and in the VulF vulval cell. Inset shows an enlarged view of VulF. (E and F) bam-2 is expressed from mid-L4 in VulF (open arrowheads). Lateral views show overlay of VulF GFP fluorescence (delta-xbaIp:bam-2::gfp) (E) on a differential interference contrast image of the same L4 worm (F). (G and H) delta-xbaIp:bam-2::gfp expression in the toroidal-shaped VulF cell [(G), ventral view] and in an adult worm [(H), lateral view]. All images except (E) and (F) are flattened confocal Z-series. Anterior is to the left. Scale bars, 10 um. | |||
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| Name | F3.large.jpg | ||||
| Depict | Expr_pattern | Expr2767 | |||
| Anatomy | WBbt:0005300 | ||||
| WBbt:0005785 | |||||
| WBbt:0006751 | |||||
| WBbt:0006759 | |||||
| WBbt:0006768 | |||||
| Acknowledgment | Template | From <Article_URL>. Reprinted with permission from <Publisher_URL>. Readers may view, browse, and/or download material for temporary copying purposes only, provided these uses are for noncommercial personal purposes. Except as provided by law, this material may not be further reproduced, distributed, transmitted, modified, adapted, performed, displayed, published, or sold in whole or in part, without prior written permission from the publisher. | |||
| Publication_year | 2003 | ||||
| Article_URL | DOI | id | 10.1126/science.1089163 | ||
| Journal_URL | Science | ||||
| Publisher_URL | AAAS | ||||
| Reference | WBPaper00006123 |
