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| WBPicture0000011101 | Description | Figure 4. Expression of egl-3 PC2. Anti-egl-3 PC2 antibody was used to stain transgenic animals expressing KP#454, a full-length rescuing EGL-3:: GFP construct (A-C). Endogenously expressed EGL-3 was visualized by staining non transgenic wild-type animals (D) or egl-3(nu349) mutants (E). Expression of egl-3 PC2 in the command neurons was examined by staining transgenic animals containing both KP#454 and nuIs24, a GLR-1:: GFP transgene, with anti-egl-3 PC2 and anti-glr-1 GluR antibodies (B). A, Expression of EGL-3:: GFP was found in the cell bodies of many neurons in the head and in ganglia in the tail (TG). In addition, axons in the nerve ring (NR) stained brightly with the anti-egl-3PC2 antibody. B, Expression of EGL-3:: GFP in the PVC command neurons was documented by double staining with anti-egl-3 PC2 (left) and anti-glr-1 GluR (middle) antibodies. The merged image is shown on the right. This panel shows a mosaic animal in which one PVC neuron (indicated by the asterisk) expressed both GLR-1 (green) and EGL-3 (red), whereas the second PVC neuron (indicated by the arrowhead) expressed GLR-1 but not EGL-3. Similar results were obtained documenting expression of EGL-3:: GFP in AVB and AVD (data not shown). D, E, Endogenously expressed EGL-3 was stained with anti-egl-3 PC2 antibodies. In wild-type animals (D), bright staining was observed in the nerve ring (NR) and ventral cord (data not shown) axons. Cell bodies (indicated by arrows) stained very weakly. In egl-3(nu349) mutants (E), the axons of the nerve ring (NR) and ventral cord (data not shown) were poorly stained, whereas bright staining of neuronal cell bodies was observed (arrows). Scale bars, 10 um. | |
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| Name | F4.large.jpg | ||
| Depict | Expr_pattern | Expr1815 | |
| Anatomy | WBbt:0005300 | ||
| WBbt:0005840 | |||
| WBbt:0006749 | |||
| WBbt:0006751 | |||
| WBbt:0006759 | |||
| Acknowledgment | Template | WormBase thanks <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL>. | |
| Publication_year | 2001 | ||
| Journal_URL | TheJournalofNeuroscience | ||
| Publisher_URL | SocietyofNeuroscience | ||
| Reference | WBPaper00004977 |
