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WBPicture0000012468DescriptionFigure 1. MISC-1::GFP reporter expression and its subcellular localization during C. elegans development. (A-B) GFP image and GFP/ DIC overlay of MISC-1::GFP expression in an early embryo. Expression is in intestinal precursor cells and has a typically mitochondrial sub-cellular localization. (C-D) GFP image and GFP/DIC overlay of reporter expression in a newly hatched L1 animal. Expression can be observed in the intestine (arrowhead points to one typical cell) and in four head neurons (arrows). As in (A,B), the GFP reporter also has a string-like appearance typical of mitochondria. (E-F) GFP image and GFP/DIC overlay of reporter expression in an adult. Expression is strongest in the pharyngeal corpus. (G) Muscles of a MISC-1::GFP adult. MISC-1::GFP co-localizes with muscle mitochondria stained with the dye Mitotracker CMXRos (MT), as seen in the overlay. (H) Seam cell syncytium of a MISC-1::GFP adult. Mitochondria were stained with Mitotracker CMXRos and nuclei were counterstained with DAPI. The overlay shows the alternating pattern of mitochondria and nuclei in the seam cell syncytium, and illustrates how MISC-1::GFP co-localizes with the mitochondria.
NameFigC.jpg
DepictExpr_patternExpr9243
AnatomyWBbt:0003679
WBbt:0003681
WBbt:0005772
Cellular_componentGO:0005739
AcknowledgmentTemplateReprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>.
Publication_year2011
Article_URLDOIid10.1371/journal.pone.0017827
Journal_URLPLoSOne
Publisher_URLPLoS
ReferenceWBPaper00038273