WormBase Tree Display for Picture: WBPicture0000013076
expand all nodes | collapse all nodes | view schema
| WBPicture0000013076 | Description | Figure 6 Native UNC-16 immunolocalization in C. elegans whole mounts. (A) Representative, identically scaled images of UNC-16 immunoreactivity (Red) in wild-type and unc-16(ce483) motor neuron cell somas in the ventral nerve cord. Note that the red signal is greatly diminished or absent in the unc-16 mutant, thus demonstrating that the red signal represents UNC-16. Each strain also contains the ceIs123 integrated transgene, which expresses soluble GFP in a subset of nine motor neurons. The top images show UNC-16 signal only. The bottom images show an overlay of the UNC-16 and GFP signals to demonstrate equal permeablization of wildtype and mutant animals. GFP protein (green) was detected using a Far Red secondary antibody to avoid native GFP fluorescence. (B) UNC-16 appears to be present in most or all neurons. The image shows UNC-16 protein in the retrovesicular ganglion in the animal's head. Note that the staining shows up as small puncta, with some areas of heavy concentration. (C) UNC-16 protein in the isolated neuron ALM. Because this neuron is well separated from other neurons the initial segment of the process is visible. UNC-16 appears most heavily concentrated in the cell soma and the initial segment of the process, but it can also be found sparsely throughout the length of the process. A green line adjacent to the process marks its path. (D) The synaptic regions of axons also contain UNC-16. Representative, identically scaled images of UNC-16 protein (Red) in wild-type and unc-16(ce483) motor neuron axons in the dorsal nerve cord. Note that the red signal is greatly diminished or absent in the unc-16 mutant, thus demonstrating that the red signal represents UNC-16. Each strain also contains the ceIs123 integrated transgene, which expresses soluble GFP in a subset of nine motor neurons. Some UNC-16 signal remains in the mutant since the region used to produce the antibody contains sequences upstream of the nonsense mutation. The top images show UNC-16 signal only and is an area of exceptionally uniform concentration of UNC-16 in the center of the image. A more typical pattern is shown in E. The lower images show an overlay of the UNC-16 and GFP signals to demonstrate equal permeablization of wild-type and mutant animals. GFP protein (green) was detected using a Far Red secondary antibody to avoid native GFP fluorescence. (E) UNC-16 protein in the synaptic region of axons typically contains sparse areas of heavy concentration interspersed with large areas containing a low concentration of UNC-16. Shown is a 74-mm region of the dorsal cord. The top image shows UNC-16 signal only (red). The bottom image shows signals for synaptic vesicle clusters (green) and active zones (blue) overlaid on the UNC-16 signal. | |||
|---|---|---|---|---|---|
| Name | F6.large.jpg | ||||
| Depict | Expr_pattern | Expr11021 | |||
| Anatomy | WBbt:0005735 | ||||
| Cellular_component | GO:0030424 | ||||
| GO:0043025 | |||||
| Acknowledgment | Template | WormBase thanks the journal <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL> | |||
| Publication_year | 2013 | ||||
| Article_URL | DOI | id | 10.1534/genetics.112.147348 | ||
| Journal_URL | Genetics | ||||
| Publisher_URL | GSA | ||||
| Reference | WBPaper00041921 |
